Detecting instrument on capillary cataphoretic-multi-photon excitation fluorescent column

Abstract

The invention discloses a column detector of capillary electrophoresis-multiphoton exciting fluorescence. It contains semiconductor laser, objective lens, computer, collecting card, electron-multiplier phototube, dichroscope, band-pass filter, all mirror, platinum electrode, constant-current high-voltage power, first buffer pool, second buffer pool and column detector. Connects square and round capillaries, in which round capillary is used for division (according to experiments, division effect of round capillary is batter than that of squire capillary) and squire capillary is used for column detecting. The combination of squire and round capillaries not only simplifies experimental devices, lowers cost and simplifies complex post column detection, but also improves detecting sensitivity.

Description

technical field [0001] The invention belongs to the high-sensitivity multi-photon fluorescence detection technology, in particular to a capillary electrophoresis-multi-photon excitation fluorescent column detector. Background technique [0002] Capillary electrophoresis (high-performance capillary electrophoresis, HPCE), as a separation and analysis technique, has the advantages of high separation efficiency, short analysis time and small injection volume, and is easy to operate and easy to automate. With the diversification of separation modes and the innovation of detection technology, capillary electrophoresis has been widely used in the fields of life science, environmental health and medicine. [0003] As a new detection technology, Multi-photon Excited Fluorescence (MPF) technology has the advantages of small excitation volume (10 -18 L), less photodamage and phototoxicity (using near-infrared laser), high sensitivity (zmol-ymol level detection limit) and low backgrou...

AI Technical Summary

Problems solved by technology

The difficulties in post-column detection are: i), there is a focus point problem in post-column detection, and the focus of the objective lens is aligned with the center of the capillary outlet, and CCD detection is used, which increases the equipment cost of the detection system, and the experimental operation becomes complicated; ii) 1. There is a problem of debris accumulation in the post-column detection. The substances flowing out of the capillary in the experiment are easy to accumulate at the detection window, which is not conducive to excitation and fluorescence detection; iii) There is a cleaning problem in the post-column detection. Since one end of the capillary is the detection window, This complicates the cleaning of the capillary, and the detection end of the capillary must be moved to the cleaning pool, resulting in secondary focusing problems

In short, the traditional detection device adopts complex post-column detection, the experimental operation is cumbersome, the cost is high, and the repeatability is poor, and the sensitivity is also affected

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