Method for preparing poultry cholera microcapsule vaccine

A technology of microcapsules and fowl cholera, applied in the field of bioengineering, achieves simple and reasonable effects, high antigen content, and convenient use

Inactive Publication Date: 2007-10-31
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a preparation method of fowl cholera microcapsule vaccine for the existing shor...

Method used

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  • Method for preparing poultry cholera microcapsule vaccine
  • Method for preparing poultry cholera microcapsule vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] After rejuvenating the freeze-dried strains of Pasteurella multocida type A, select typical colonies and culture them to make seed liquid, use the flat bottle solid culture method to obtain semi-finished products with a bacterial count of more than 12 billion / ml, and pass the inactivation test Then mix with sodium alginate solution for primary emulsification, then mix this emulsion with chitosan solution for secondary emulsification, dehydrate the final emulsion through a spray dryer, and make microcapsule vaccine dry powder, each gram of vaccine contains bacteria The body size is 350 billion, and the vaccine particle size ranges from 2-9um (see Figure 1). The test chickens produced an immune response 3 days after being inoculated with the vaccine, and within 6 months of immunization, the average protection rate was 80.67%.

Embodiment 2

[0017] After the freeze-dried strain of Pasteurella multocida type A is rejuvenated, typical colonies are selected and cultured to make seed bacterial liquid, and the semi-finished product containing more than 9 billion bacteria / ml is obtained by deep aerated fermentation culture method, which is tested by inactivation Then mix with sodium alginate solution for primary emulsification, then mix this emulsion with chitosan solution for secondary emulsification, dehydrate the final emulsion through a spray dryer, and make microcapsule vaccine dry powder, each gram of vaccine contains bacteria The body size is 300 billion, and the vaccine particle size ranges from 1-12um (see Figure 2). The test chickens produced an immune response 3 days after being inoculated with the vaccine, and within 6 months of immunization, the average protection rate was 76.32%.

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Abstract

The invention discloses a preparing method of cholera fowl microcapsule vaccine, which comprises the following steps: recovering and passing A type multiple killing property pasteurellosis freeze-drying bacterial; producing seed bacterial liquid; culturing; breeding; getting half-finished product with bacterial number above 9 billion/ml; deactivating the half-finished product bacteria liquid; testing; proceeding isotrope with equivoluminal 2% sodium alginate solution through dispersing device; proceeding first emulsify; proceeding twice emulsify with the emulsion and equivoluminal 5% chitose solution through high pressure emulsifying device; dewatering the final emulsion through spray dryer; producing microcapsule vaccine dry powder. This method possesses scientific method, high antigen content, long slow release time, low toxic side action and low cost.

Description

technical field [0001] The invention relates to a preparation method of fowl cholera microcapsule vaccine, which belongs to the technical field of bioengineering. Background technique [0002] Fowl cholera is a contact septic infectious disease caused by Pasteurella multocida that mainly attacks poultry (chickens, ducks, geese) and wild birds, with high morbidity and mortality. Among the measures to prevent fowl cholera, in addition to keeping the breeding environment clean, vaccine prevention has always been the main method to prevent and control fowl cholera. Although my country currently has vaccine products for the prevention of fowl cholera such as "fowl cholera propolis inactivated vaccine" and "G 190 Freeze-dried live vaccine " etc., but these vaccines still have many deficiencies: the one, cause various stress reactions (injection local inflammation, muscle necrosis, food and egg reduction, growth inhibition, etc.) after inactivated vaccine injection; Because some ...

Claims

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Application Information

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IPC IPC(8): A61K39/102A61K9/50A61P31/04
Inventor 刘学贤龚建森吕晓娟
Owner JIANGSU INST OF POULTRY SCI
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