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Method for determining phase change temperature of liposome

A phase transition temperature, liposome technology, applied in the field of analysis and testing, can solve the problems of complex operation and low sensitivity, and achieve the effect of avoiding interference, convenient, fast and accurate measurement

Inactive Publication Date: 2007-11-28
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the shortcomings of low sensitivity and complex operation of the existing method for measuring the phase transition temperature of liposomes, the present invention provides a method for determining the phase transition temperature, which effectively overcomes the shortcomings of the prior art

Method used

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  • Method for determining phase change temperature of liposome
  • Method for determining phase change temperature of liposome
  • Method for determining phase change temperature of liposome

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: prepare berberine hydrochloride liposome (100% lecithin) suspension, the liposome suspension that will make is on centrifuge with 12000r min -1 Centrifuge for 30min, remove the supernatant, and keep the lower layer of liposomes. Weigh about 10.0 mg and place it in a differential scanning calorimeter at 2°C min -1 Heating rate detection. According to the DSC spectrum (see Figure 1), a large endothermic peak appears at 60.6°C.

[0030] Select 25°C, 37°C, 50°C, 60°C, 70°C, and 80°C to prepare berberine hydrochloride liposomes at 6 incubation temperatures. After dialysis with a dialysis bag for 12 hours, dialyze the external fluid to a volume of 100mL. Under 345nm, measure the absorbance of solution with ultraviolet spectrophotometry, convert the concentration of solution by standard curve (see Fig. 2), the relationship between the drug encapsulation efficiency of liposome and incubation temperature is shown in Fig. 3. The results showed that the encapsulat...

Embodiment 2

[0031] Embodiment 2: prepare berberine hydrochloride liposome (lecithin and cholesterol mass ratio is 3 to 1) suspension, the liposome suspension that will make is on centrifuge with 12000r min -1 Centrifuge for 30min, remove the supernatant, and keep the lower layer of liposomes. Weigh about 10.0 mg and place it in a differential scanning calorimeter at 2°C min -1 Heating rate detection. According to the DSC spectrum (see Figure 4), a large endothermic peak appears at 41.8°C.

[0032] Select 39°C, 41°C, 43°C, and 45°C to prepare berberine hydrochloride liposomes at four incubation temperatures. After 12 hours of dialysis with a dialysis bag, dialyze the external fluid to 100mL at a constant volume, and use ultraviolet spectroscopy at 345nm. Photometer measures the absorbance of solution, and converts the concentration of solution by standard curve (see Fig. 2), and the relationship between the drug encapsulation efficiency of liposome and incubation temperature is shown in ...

Embodiment 3

[0033] Embodiment 3: prepare berberine hydrochloride liposome (lecithin and cholesterol mass ratio is 5 to 1) suspension, the liposome suspension that will make is on centrifuge with 12000r min -1 Centrifuge for 30min, remove the supernatant, and keep the lower layer of liposomes. Weigh about 10.0 mg and place it in a differential scanning calorimeter at 2°C min -1 Heating rate detection. According to the DSC spectrum (see Figure 6), a large endothermic peak appears at 55.8°C.

[0034] Select 47°C, 50°C, 53°C, 56°C, and 59°C to prepare berberine hydrochloride liposomes at 5 incubation temperatures, dialyze with a dialysis bag for 12 hours, dialyze the external fluid to 100mL at a constant volume, Measure the absorbance of the solution with a UV spectrophotometer, and convert the concentration of the solution through a standard curve (see Figure 2), and the relationship between the drug encapsulation efficiency of the liposome and the incubation temperature is shown in Figure...

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Abstract

The invention relates to a method of measuring transformation temperature of liposome, belonging to the analysis measuring field. The method is that while transformation temperature of some liposome is measured by DSC the relation curve of drug loading efficiency and incubation temperature; according to the incubation temperature corresponding to the highest peak of drug loading efficiency, actual transformation peak, namely accurate transformation temperature of the liposome can be determined in many heat absorption peaks in DSC map. The method can determine transformation temperature of liposome more accurately. If transformation point of some liposome is adjusted furthermore, only DSC is used to track the change of transformation peak and interference from other mixed peaks is avoided to measure the phase transformation of liposome quickly, conveniently and accurately.

Description

technical field [0001] The invention belongs to the field of analysis and testing, in particular to a method for measuring the phase transition temperature of liposomes. Background technique [0002] After the drug is combined with the carrier to form a drug carrier system, the absorption and distribution of the drug are no longer determined by the drug itself, but are affected by the physical and chemical properties of the carrier. Selecting the appropriate drug carrier material according to clinical requirements can not only deliver the drug to the target organ, but also play a beneficial role in the physicochemical properties and pharmacological activity of the drug. As the carrier of drug delivery and controlled release, liposome constitutes a new drug controlled release system. This kind of carrier can not only improve the dissolution rate of the drug, facilitate the absorption of the drug, but also enable the drug to reach the target site quickly, and is especially su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N25/02G01N33/15G01N33/483
Inventor 安学勤张敏裘丹
Owner NANJING NORMAL UNIVERSITY
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