Method for preparing paeonol and paeoniflorin from cortex moutan

[0025] Example one

[0026] Take 500kg of decoction pieces of Danpi, dry for 6 hours at 50℃, soak in water for 24 hours, reflux for extraction, collect the distillate to 12 times the volume (6L), freeze at -4℃ for 8 hours, take it out and thawed immediately and filter with suction. The insoluble substance is Dan Dermatol. Add 10 times the amount of 70% ethanol aqueous solution to the dregs and heat and reflux for two extractions, 0.5 hours each time. The extracts are filtered, combined, and concentrated under reduced pressure to 1g/mL (crude drug amount), top D 201 Macroporous resin (0.2-0.6g crude medicinal materials/g wet resin). Rinse with 4BV deionized water, discard, continue to purify with 4BV 20% ethanol aqueous solution, discard, and then eluate with 4BV 40% ethanol aqueous solution, collect the eluate, concentrate under reduced pressure to alcohol-free, freeze-dry, and then obtain Paeonia lactiflora Glycoside part. The extraction rate of paeonol medicinal materials is 2.06% and the medicinal material extraction rate of paeoniflorin is 0.32% through liquid phase determination.

[0027] Example two

[0028] Take 500kg of decoction pieces of Danpi, dry for 6 hours at 50℃, soak in water for 10 hours, reflux for extraction, collect the distillate to 8 times the volume (4L), freeze at -4℃ for 4 hours, take out and melt and filter immediately, the insoluble substance is Dan Dermatol. Add 10 times the amount of 30% ethanol aqueous solution to the dregs and extract once by heating and refluxing for 0.5 hours each time. The extracts are filtered, combined, and concentrated under reduced pressure to 1g/mL (crude drug amount), top D 201 Macroporous resin (0.2-0.6g crude medicinal materials/g wet resin). Rinse with 2BV deionized water, discard, continue to purify with 3BV 10% ethanol aqueous solution, discard, and then eluate with 3BV 30% ethanol aqueous solution, collect the eluate, concentrate under reduced pressure to alcohol-free, freeze-dry, and then obtain peony Glycoside part. The extraction rate of paeonol medicinal material is 1.89% by liquid phase determination, and the medicinal material extraction rate of paeoniflorin is 0.30%.

[0029] Example three

[0030] Take 500kg of decoction pieces of Danpi, dry for 6 hours at 50°C, soak in water for 15 hours, reflux for extraction, collect the distillate to 12 times the volume (6L), freeze at -4°C for 6 hours, take out and melt and immediately filter with suction. The insoluble substance is Dan Dermatol. Add 8 times the amount of 50% ethanol aqueous solution to the dregs and heat and reflux for two extractions for 0.5 hours each time. The extracts are filtered, combined, and concentrated under reduced pressure to 1g/mL (crude drug amount), top D 201 Macroporous resin (0.2-0.6g crude medicinal materials/g wet resin). Rinse with 3BV deionized water, discard, continue to purify with 3BV 20% ethanol aqueous solution, discard, and then eluate with 3BV 50% ethanol aqueous solution, collect the eluate, concentrate under reduced pressure to alcohol-free, freeze-dry, and then obtain peony Glycoside part. The extraction rate of paeonol medicinal materials was 1.92% and the medicinal material extraction rate of paeoniflorin was 0.31% through liquid phase determination.