Method for expanding uterus intima cell externally on a large scale

Abstract

The invention provides a method for separating and purifying endometrial stromal cell and proceeding with large scale external culture, which improves the external purifying ratio and the breeding ability of endometrial stromal cell, adjusts the proliferative stage of endometrium and uterus physiology by treating adenotropic enthusiasm hormone and adenotropic ovulation hormone for the experimental animal, purifies the endometrial stromal cell with centrifuge method, establishes an external culture system with estrogen and gestagen in the cell purification and breeding, improves the breeding ability of the endometrial stromal cell, achieves a large scale breeding purpose and provides the abundant seed cell for the construction of the organizing engineer uterine tissue.

Description

technical field

[0001] The invention belongs to the field of tissue engineering and relates to a biotechnological method for large-scale expansion of endometrium epithelial cells and stromal cells in vitro. This method can effectively isolate, purify and expand endometrial cells. Background technique

[0002] Tissue engineering is the application of the principles and technologies of life science and engineering, on the basis of a correct understanding of the relationship between the structure and function of mammals in both normal and pathological states, research and development for the repair, maintenance, and promotion of various human tissues or The science of functional and morphological biological substitutes after organ injury. As an important reproductive organ of female mammals, the uterus plays an important role in the study of fetuses and various pathological and reproductive mechanisms. The physiological functions of the uterus are mainly performed by the endo...