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Method for expanding uterus intima cell externally on a large scale

An endometrium and in vitro amplification technology, which is applied in the field of tissue engineering, can solve the problems of cumbersome steps, influence on cell viability, and high cost, and achieve the effects of facilitating separation and collection, improving proliferation ability, and facilitating adherent growth

Inactive Publication Date: 2008-01-02
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

Although the purity of the endometrial epithelial cells separated by filtration is high, the method is cumbersome and easy to contaminate, causing large cell loss and affecting cell viability.
However, the immunomagnetic bead method has problems such as high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] The present invention will be further described in conjunction with specific embodiments. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the invention.

[0015] A specific example taken by the present invention is the separation and purification of rabbit endometrial cells:

[0016] New Zealand white rabbits of 2-2.5Kg are used as experimental animals. 100 IU PMSG was injected intramuscularly, 75 IU hCG was injected into the ear vein 24 hours later, 5ml of air was injected into the ear vein 18 hours later, the abdomen was disinfected, the abdominal cavity was opened, bilateral uteri were taken, and they were placed in D’Hanks balance solution. Gently scrape the intima with a scalpel in the ultra-clean workbench, add 0.01-0.25% trypsin to digest at 37°C for 2-5min, stop the digestion with serum-containing DMEM / F12 culture medium, centrifuge, and use 10ml serum-containing DMEM / F12 medium to resu...

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Abstract

The invention provides a method for separating and purifying endometrial stromal cell and proceeding with large scale external culture, which improves the external purifying ratio and the breeding ability of endometrial stromal cell, adjusts the proliferative stage of endometrium and uterus physiology by treating adenotropic enthusiasm hormone and adenotropic ovulation hormone for the experimental animal, purifies the endometrial stromal cell with centrifuge method, establishes an external culture system with estrogen and gestagen in the cell purification and breeding, improves the breeding ability of the endometrial stromal cell, achieves a large scale breeding purpose and provides the abundant seed cell for the construction of the organizing engineer uterine tissue.

Description

technical field [0001] The invention belongs to the field of tissue engineering and relates to a biotechnological method for large-scale expansion of endometrium epithelial cells and stromal cells in vitro. This method can effectively isolate, purify and expand endometrial cells. Background technique [0002] Tissue engineering is the application of the principles and technologies of life science and engineering, on the basis of a correct understanding of the relationship between the structure and function of mammals in both normal and pathological states, research and development for the repair, maintenance, and promotion of various human tissues or The science of functional and morphological biological substitutes after organ injury. As an important reproductive organ of female mammals, the uterus plays an important role in the study of fetuses and various pathological and reproductive mechanisms. The physiological functions of the uterus are mainly performed by the endo...

Claims

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Application Information

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IPC IPC(8): C12N5/06C12N5/08C12N5/071
Inventor 王常勇王海滨江红郭希民段翠密王和平宋宇轩
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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