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Method for preparing inactivated vaccine both for preventing chicken Newcastle disease and infectious bronchitis

A technology for double inactivated vaccine and bronchitis, which is applied in the field of preparation of double inactivated vaccine, can solve the problems of harm to the chicken industry, high cost of epidemic prevention, inconvenient use, etc. specific effect

Inactive Publication Date: 2008-01-09
HENAN AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0002] Chicken Newcastle disease, chicken respiratory type and renal type infectious bronchitis are very harmful to the chicken industry. At present, there are only corresponding single inactivated vaccines, such as chicken Newcastle disease inactivated vaccine and infectious bronchitis (unit price) inactivated vaccine, which need to be separated separately Injection, very inconvenient to use, high cost of epidemic prevention

Method used

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Embodiment Construction

[0021] The preparation method of the double inactivated vaccine of prevention chicken Newcastle disease, infectious bronchitis, selects virus seed to be Newcastle disease virus La Sota strain, infectious bronchitis virus M 41 and HN 99 For kidney-type mutant strains, the steps to prepare the dual vaccine are as follows:

[0022] 1. The source of poisonous seeds

[0023] (1) La Sota strain was used for chicken Newcastle disease, and the virulence test was F 48 E. 9 , provided by Henan Agricultural University. The standards and storage methods of the virus seeds are the same as those specified in item 1 of the "Regulations for the manufacture and inspection of chicken Newcastle disease low-virulence live vaccines" in the "Regulations of the People's Republic of China for Veterinary Biological Products" (hereinafter referred to as "the regulations").

[0024] (2) Infectious bronchitis is manufactured and inspected using standard poisonous M 41 HN 99 strains were provided by...

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Abstract

The invention is concerned with a kind of bacterin to prevent fowl infectious disease, relating to a kind of bacterin preparation method to prevent Newcastle Disease and infectious bronchitis. The selected genus is Newcastle Disease virus La Sota individual plant, infectious bronchitis virus M41 individual plant and HN99 kidney type of aberrance individual plant. Dilute two kind of virus and inoculate in chick embryo allantoic cavity, collect liquid of embryo to alive and dead embryos as seed. Dilute and inoculate in allantoic cavity of affectable chick embryo to get liquid of virus embryo. Concentrate with equipment and add with formaldehyde solution to prepare bacterin through emulsion process. This invention can prevent Newcastle Disease, chick kidney type and breathing type infectious bronchitis, and it can reduce the times of injection and the cost of epidemic prevention for easy using and practicality.

Description

technical field [0001] The invention relates to a vaccine for preventing poultry infectious diseases, in particular to a preparation method of a dual inactivated vaccine for preventing Newcastle disease and infectious bronchitis. Background technique [0002] Chicken Newcastle disease, chicken respiratory type and renal type infectious bronchitis are very harmful to the chicken industry. At present, there are only corresponding single inactivated vaccines, such as chicken Newcastle disease inactivated vaccine and infectious bronchitis (unit price) inactivated vaccine, which need to be separated separately Injection is inconvenient to use and the cost of epidemic prevention is high. Contents of the invention [0003] The purpose of the invention is to provide a preparation method of a dual inactivated vaccine capable of preventing chicken Newcastle disease, respiratory type and renal type infectious bronchitis. [0004] The present invention adopts to realize above-mention...

Claims

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Application Information

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IPC IPC(8): A61K39/215A61K39/12A61P11/00
Inventor 崔保安舒德广王莉徐端红高文明王学斌张素梅魏占勇
Owner HENAN AGRICULTURAL UNIVERSITY
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