Reagent kit for detecting epidermal growth factor acceptor third type mutant by real-time fluorescence quantitative PCR

An epidermal growth factor, real-time fluorescence quantitative technology, applied in fluorescence/phosphorescence, microbial determination/inspection, biochemical equipment and methods, etc., can solve problems such as disputes over the expression level of EGFRvIII, and achieve credible results and quantitative accuracy. High, improve work efficiency
CN101130818AInactive Publication Date: 2008-02-27ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2008-02-27
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The present invention relates to a kit. Said kit can use cDNA obtained by means of reverse transcription of mRNA sample as template, and combines it with real-time fluorescent PCR detection technique, so that it can be mainly used for determining expression level of epidermal growth factor receptor III type mutant mRNA in various tumor tissues.
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Description

technical field

[0001] The invention belongs to the field of biological technology, and is a kit for accurately and quantitatively detecting the mRNA expression of human epidermal growth factor receptor type III mutant through real-time fluorescent quantitative PCR detection technology. Background technique

[0002] Epidermal Growth Factor Receptor variant III (EGFRvIII) gene is derived from the in-frame deletion mutation at positions 276-1075 in the normal EGFR cDNA nucleotide sequence, corresponding to the 2nd to 7th outer positions of the mRNA sequence Deletion of exons. Its formation is caused by the rearrangement of the epidermal growth factor receptor (Epidermal Growth Factor Receptor, EGFR) gene amplification process, which has a certain relationship with the increase of EGFR mRNA expression. EGFRvIII can form dimers and self-phosphorylate independently of ligands such as EGF, which eventually leads to the constitutive activation of unregulated kinases, stimulates ce...

Claims

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