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Siberia lily detoxification test tube plantlet squama bulb highly-efficient regeneration method

A high-efficiency regeneration, test-tube seedling technology, applied in the field of tissue culture in biological science, can solve problems such as the differentiation of indefinite bud-like microbulbs in longitudinal slices without scales, and achieve the effect of improving efficiency and reproduction coefficient.

Inactive Publication Date: 2008-03-05
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since Chen Weimin and Huang Jiming respectively used lily scales as explants to successfully induce the regeneration of lily plants in 1982, there have been many reports on the tissue culture of lily scales at home and abroad. Regenerated plants, but there is no report on the differentiation of adventitious bud-like microbulbs in longitudinal slices of scales

Method used

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  • Siberia lily detoxification test tube plantlet squama bulb highly-efficient regeneration method
  • Siberia lily detoxification test tube plantlet squama bulb highly-efficient regeneration method
  • Siberia lily detoxification test tube plantlet squama bulb highly-efficient regeneration method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Select a test tube bulb with a diameter of 1 to 1.5 cm, peel off the outer 1 to 3 layers of scales, and use a sharp knife to cut the scales into thin slices with a thickness of 1 to 2 mm under sterile conditions, and each scale can obtain 6 to 8 pieces on average. The scales are sliced ​​longitudinally, and the shape of the slices is shown in Figure 2.

Embodiment 2

[0047] In the induction medium, the effect of 2,4-D addition concentration on the differentiation of adventitious bud-like microbulbs in the longitudinal slices of scales is shown in Table 1.

[0048] Table 1 The effect of 2,4-D concentration on the differentiation of adventitious bud-like microbulbs in longitudinal slices of scales

[0049]

[0050] The longitudinal slices of the scales were inoculated on the induction medium for five days and gradually turned green. Ten days later, buds appeared at the base, and the epidermis of the adaxial and abaxial surfaces were slightly callused. Bud points appeared in the slightly callused epidermis in 12 days, and complete adventitious bud-shaped miniature bulbs were formed in 15 days. Table 1 shows that when the dosage of TDZ is 0.005mg / L, the average number of adventitious bud-like microbulbs increases with the increase of the dosage of 2,4-D, the maximum is at 0.5mg / L, and then gradually decreases. The occurrence freque...

Embodiment 3

[0052] In the induction medium, the effects of different concentrations of TDZ on the differentiation of adventitious bud-like microbulbs from scale longitudinal sections are shown in Table 2 and Figure 6.

[0053] Table 2 Effects of different concentrations of TDZ on differentiation of adventitious bud-like microbulbs in longitudinal slices of scales

[0054]

TDZ concentration

(mg / L)

Inoculation outside

Number of implants

(indivual)

Regenerated Adventitious Bud Micro

Bulb explant number

(indivual)

adventitious bud-like microscale

stem frequency

(%)

average adventitious bud

number of miniature bulbs

(indivual)

total microbulbs

number

(indivual)

0

0.001

50

50

43

48

86

96

4.04

5.75

173

276

[0055] 0.002

0.004

0.005

0.006

0.008

0.01

0.05

50

50

50

50

50

50

50

43 ...

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Abstract

The present invention relates to a high-effective bulb regeneration method by using Siberian lily sterilized test-tube seedling lepis. It is characterized by that said method includes the following steps: selecting test-tube bulb whose diameter is 1-1.5cm, utilizing its outside 1-3 layers of lepis, under the sterile condition cutting said lepis along its longitudinal direction to obtain thin slice whose thickness is 1-2mm, inoculating said thin slice on the induction culture medium to induce generation of adventitious bud, when the adventitious bud is grown to 2-3cm, placing it into bulb culture medium to make culture, and when the bulb diameter is grown to 1.5-2.0cm, making cold-storage culture, then transplanting said bulb into the matrix formed from perlite and turf earth to make conventional seedling cultivation.

Description

1. Technical field [0001] The invention belongs to the technical field of tissue culture in biological sciences, and in particular relates to a technique for efficiently regenerating test tube miniature bulbs from lily test tube seedling scales. 2. Background technology [0002] Lily is one of the top five fresh-cut flowers in the world. It is not only beautiful in appearance, rich in color and fragrant in smell, but also has edible and medicinal value in its bulbs. Therefore, in recent years, the research on lily has become more and more in-depth and extensive, especially in the aspects of tissue culture and rapid propagation, which have become mature and industrialized. Siberia lily (Siberia) belongs to the oriental lily hybrid line, and is one of the most popular lily varieties in domestic and foreign flower markets in recent years. [0003] Traditional lily propagation methods mainly adopt conventional bulb division, bulbil division, scale cutting, scale embedding and s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 施季森席梦利王鹏凯胡凤荣
Owner NANJING FORESTRY UNIV
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