Reagent case for lung carcinoma susceptibility detection through PARP1 gene

A kit and susceptibility technology, applied in the field of kits for detecting lung cancer susceptibility through PARP1 gene, can solve the problems of reducing enzyme activity and increasing the risk of occurrence

Inactive Publication Date: 2008-03-12
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Molecular biology studies have shown that amino acid 762 on PARP1 is converted from Val to Ala, which reduces the activity of the enzyme and leads to an increased risk of various cancers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1. Use of detection kit

[0024] Step 1: Extraction of DNA template

[0025] The genomic DNA of oral epithelial cells was extracted by silica gel adsorption method.

[0026] Step 2: Fluorescence quantitative PCR reaction

[0027] Use a fluorescent quantitative PCR detection kit that can detect lung cancer susceptibility, which contains the following primer pairs and fluorescent probes:

[0028] Sense primer: 5'-TTTGCCATTCACTGTGTTGG-3' (SEQ ID NO: 2) Tm value is 58°C

[0029] Antisense primer: 5'-ATCCTTGCTGCTATCATCA-3' (SEQ ID NO: 3) Tm value is 54°C

[0030] Fluorescent probe: 5'-CAGGCCAAGGCGGAAATGCT-3' (SEQ ID NO: 4) Tm value is 64°C

[0031] The fluorescence quantitative PCR reaction system is a total volume of 10μl, containing 2μl of DNA template at a concentration of 20ng / μl, 1μl of 10X fluorescence quantitative PCR reaction buffer, 0.1μl of 25mM dNTP mixture, 0.6μl of 25mM MgCl 2 Solution, 0.025μl (5units / μl) Taq DNA polymerase, 20μM sense primer and antisense ...

Embodiment 2

[0035] Example 2. Services to guide people to take the initiative to prevent lung cancer

[0036] At present, this service has been carried out in Shanghai Zhujian Biological Engineering Co., Ltd.

[0037] Step 1: DNA extraction

[0038] Conduct pre-service consultations for the tested persons, who will sign an informed consent form and fill in a questionnaire about eating habits. Use the oral sampling swab to sample oral epithelial cells according to the instructions in the sampling box, and use the silica gel adsorption method for DNA extraction of oral epithelial cells.

[0039] Step 2: Genotyping testing

[0040] Using the kit provided by the present invention, the rs1136410 SNP site on the PARP1 gene of the DNA of the testee is detected by fluorescent quantitative PCR to determine the genotype of the SNP site.

[0041] Step 3: Instruct people to take the initiative to prevent lung cancer

[0042] Through the analysis of the SNP genotype of the tested person, a genotyping test r...

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PUM

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Abstract

The invention discloses a reagent box for detecting lung cancer susceptibility. The reagent box comprises a pair of specificity primers for detecting 161st SNP site in SEQ ID NO:1 of PARP1 gene, a specificity fluorescent probe and an ordinary assembly for fluorescent quantitative PCR detection. The reagent box predicts the susceptibility of individuals on lung cancer by detecting and analyzing the type of carried gene at SNP site of individual PARP1 gene.

Description

Technical field [0001] The invention relates to a kit for detecting disease susceptibility, in particular to a kit for detecting lung cancer susceptibility, by detecting poly(ADP-ribose) polymerase family, member 1, PARP1, also abbreviated as ADPRT) single nucleotide polymorphism (SNP) site to predict the individual's susceptibility to lung cancer. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is currently one of the most common malignant tumors around the world, and is a disease that seriously threatens human health and life. Tumor cells originate from bronchial mucosa or glands, and often have regional lymph nodes and blood metastases. Early respiratory symptoms such as irritating cough and blood in sputum are often present. The rate of disease progression is related to the biological characteristics of the cells. [0003] Over the past half century, the incidence and mortality of lung cancer in countries all over the world have increa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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