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Chinese 'Han' nationality vitiligo related mononucleotide polymorphism detection method

A technology of single nucleotide polymorphism and detection method, which is applied in the detection field of single nucleotide polymorphism related to vitiligo in Chinese Han nationality, can solve the problems such as the lack of large-scale detection of gene chips, and achieves significant results. Application value and social benefit, low cost, high precision effect

Inactive Publication Date: 2008-03-26
安徽医科大学皮肤病研究所
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Problems solved by technology

[0007] Correlation research on candidate genes: There are only sporadic reports on single nucleotide polymorphisms of a few genes in domestic and international screening of vitiligo susceptibility genes, and there is no gene chip research for large-scale detection so far

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  • Chinese 'Han' nationality vitiligo related mononucleotide polymorphism detection method
  • Chinese 'Han' nationality vitiligo related mononucleotide polymorphism detection method

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Experimental program
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Embodiment

[0086] Main reagents and instruments

[0087] Hotstar Taq DNA polymerase and dNTP were purchased from Huamei Bioengineering Company, chemical reagents were purchased from domestic chemical reagent stores, sequencing reagents and 3730 sequencer were from Applied Biosystems (ABI).

[0088] Step 1: Extraction of Genomic Deoxyribose Nucleic Acid (DNA)

[0089]Since vitiligo is a multifactorial disease involving multiple environmental factors and multiple genes, genetic heterogeneity must be considered when analyzing related genes. Choosing an isolated population with a relatively single genetic background as a material for studying polygenic diseases will help reduce the impact of genetic heterogeneity. At the same time, because these groups have a relatively fixed living environment and relatively consistent living habits, the influence of environmental factors is greatly reduced. Selecting such a group, whether it is used for linkage disequilibrium analysis or selecting famili...

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Abstract

The invention relates to the detection method for the single nucleic acid polymorphism of vitiligo pertinence in Chinese Han nationality. It forecasts the problem of clinical and curing for vitiligo sufferers by combining the SNP and pathogenic gene mutation selection. The method includes extracting DNA, doing the PCR for the DNA by using the upper primer and / or backward primer, the SNP detection to determine if the upper 197 site of G in XBP1 gene is mutated into C, X conjugated protein 1 (XBP1) gene difference in SEQ ID NO:1. The invention has simple operation, low cost, high precision and it can be detected automatically. So it can not only be used for diagnosing the Chinese Han nationality vitiligo, but improve the carrier life quality before illness. It has important application value and social effect.

Description

technical field [0001] The invention relates to a detection method for X-box binding protein 1 single nucleotide polymorphism used for Chinese Han people's vitiligo correlation. Background technique [0002] Vitiligo (vitiligo) is a polygenic disease caused by genetic factors and environmental factors. The prevalence of the disease in the general population is about 0.5% to 1%, and it plagues more than 50 million patients worldwide. The disease is an acquired depigmentation skin disease caused by the reduction or absence of skin melanocytes. . The prevalence rate of the Chinese population is 0.1% to 2%, and there are about 12 million patients. The skin lesions manifest as depigmented spots of varying numbers, sizes, and shapes. Some of them are hypopigmented spots with unclear boundaries at first, and then gradually develop into milky white depigmented spots. On the contrary, the pigment in the skin increases. [0003] Due to the unknown etiology, complex pathogenesis, a...

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Application Information

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IPC IPC(8): G01N21/33G01N21/25C12Q1/68
Inventor 张学军杨森梁燕华高敏方巧云
Owner 安徽医科大学皮肤病研究所