Device for no-mark monitoring micro-array biomolecule reaction

A technology of array biological and molecular reactions, applied in measurement devices, biological tests, analytical materials, etc., to achieve the effect of improving signal-to-noise ratio, high work efficiency, and easy operation

Inactive Publication Date: 2008-04-02
INST OF PHYSICS - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects of the existing OIRD biomolecular monitoring technology, thereby provi

Method used

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  • Device for no-mark monitoring micro-array biomolecule reaction

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Embodiment 1

[0032] Referring to FIG. 1 , a device for label-free monitoring of microarray biomolecular reactions of the present invention is fabricated.

[0033] The device consists of an incident optical path, a sample stage 6, an outgoing optical path, and a data acquisition and processing system, wherein the incident optical path includes sequentially setting a polarizer 2, a photoelastic modulator 3, and a phase shifter 4 on the optical path in front of the output light of the laser 1. and a beam expander 5; the outgoing light path is analyzed by a polarization analyzer 7 and a photoelectric signal converter 9, and the incident light beam sent from the incident light path is reflected by the sample on the sample stage 6 and passes through the described polarization analyzer 7 and the photoelectric signal in sequence A signal converter, the photoelectric signal converter is electrically connected to the data acquisition and processing system 12 . Described photoelectric signal converte...

Embodiment 2

[0041] In this embodiment, the phase shifter 4 adopts a Pokes cell of the model Cleveland Crystal IMPACT10, and the others are the same as in Embodiment 1. Compared with Embodiment 1, the sensitivity of the device in this embodiment can be improved by an order of magnitude.

Embodiment 3

[0043] In this embodiment, the pulse switch circuit 8 adopts the internal clock pulse circuit of the CCD area array. Others are the same as in Embodiment 1. Compared with Embodiment 1, the time used by the device of this embodiment to monitor the same biochip sample can be shortened by 0.5 minutes.

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Abstract

The invention provides a device for non-mark monitoring of microorganism array biomolecular reaction, comprising an incident light path, a sample table, an emergent light path and a data acquisition and processing system, wherein, the incident light path comprises a laser, a polarizer, a photoelastic modulator, a phase shifter and a beam expander arranged between the phase shifter and the sample table; the emergent light path comprises a polarization analyzer and an electrooptical signal converter; the electrooptical signal converter comprises a CCD area array, a pulse switching circuit, a first phase-locked amplifier and a second phase-locked amplifier; the output end of the CCD area array is respectively in electric connection with the input ends of the pulse switching circuit, the first phase-locked amplifier and the second phase-locked amplifier. With a simple structure and easy operation, the device reduces the noise caused by moving a sample and greatly improves signal-noise ratio; meanwhile, the device can complete non-mark quick high-flux monitoring of the reactions of the array elements of a micro array at the same time, thereby greatly increasing monitoring efficiency.

Description

technical field [0001] The invention relates to a monitoring device for microarray biomolecular reaction, in particular to a device for monitoring biomolecular reaction with high throughput and fast without label. Background technique [0002] Commonly used methods for monitoring biomolecules are fluorescent labeling and surface plasmon resonance. Fluorescent labeling is a commonly used damage monitoring method. This method has disadvantages such as complicated monitoring process, can change the structure of protein molecules, and cause photobleaching. Surface plasmon resonance is currently a commonly used label-free monitoring method for biomolecular interactions. method, this method requires biomolecular microarrays to be formed on functional gold films, and the thickness and uniformity of the gold films are highly required. [0003] Oblique-incidence reflectivity difference (OIRD) is a special high-sensitivity optical measurement technique for measuring the optical prope...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/00
Inventor 张洪艳王旭吕惠宾陆珩曹玲柱宁廷银孙志辉周岳亮金奎娟杨国桢
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
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