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Method for separating and purifying cordycepin

A technology for separation and purification of cordycepin, applied in the field of separation and purification of cordycepin, can solve the problems of high industrial production cost, poor separation and purification selectivity, low extraction rate, etc., and achieve the effects of low cost, low cost and abundant sources

Inactive Publication Date: 2008-04-09
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for separation and purification of cordycepin to solve the shortcomings of low extraction rate, poor separation and purification selectivity, and high industrial production cost in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Crude extraction of cordycepin: use 3Kg of Cordyceps militaris wheat grain culture medium as raw material, crush into coarse grains, add 10 times the weight of water, soak at room temperature for 60 minutes, heat to a slight boil, maintain this temperature for 60 minutes, and filter. Repeat the above steps for the filter residue, extract once more, and combine the filtrates.

[0016] 2. Purification of the crude extract of Cordyceps culture medium: pass the supernatant of the extract after standing overnight through NKA-II macroporous resin, so that the resin can fully absorb the cordycepin in the extract. First elute the macroporous resin with 10% ethanol to remove part of the impurities, then elute with 50% ethanol, and collect this part to concentrate and freeze-dry for subsequent use (obtaining 7g samples, the purity of cordycepin reaches 40%), and use 95% ethanol to treat the resin It is regenerated and ready for recycling.

[0017] 3. Further purification of c...

Embodiment 2

[0020] 1. Crude extraction of cordycepin: use 2Kg of Cordyceps militaris wheat grain culture medium as raw material, crush it into coarse grains, add 10 times the weight of water, soak at room temperature for 60 minutes, heat to a slight boil, maintain this temperature for 90 minutes, and filter. Repeat the above steps for the filter residue, extract once more, and combine the filtrates.

[0021] 2. Purification of the crude extract of Cordyceps culture medium: pass the supernatant of the extract after standing overnight through NKA-II macroporous resin, so that the resin can fully absorb the cordycepin in the extract. First elute the macroporous resin with 10% ethanol to remove part of the impurities, then elute with 50% ethanol, and collect the part to concentrate and freeze-dry for subsequent use (obtaining 4g sample, the purity of cordycepin reaches 30%), and use 95% ethanol to treat the resin It is regenerated and ready for recycling.

[0022] 3. Further purification of ...

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PUM

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Abstract

The invention discloses a method for separation and purification of cordycepin, the method adopts the cordyceps militaris solid drying culture media as the raw materials, and uses NKA-II macroporous resin column and C18 column for purification and finally uses the distilled water for repeated recrystallization. The purity of the obtained cordycepin can achieve more than 95 percent. The method has the advantages of rich sources of the raw materials, low cost, simple process and high purity, so the method is applicable to the industrialization production.

Description

technical field [0001] The invention belongs to the field of pharmaceutical industry, in particular to a method for separating and purifying cordycepin Background technique [0002] Cordycepin (Cordycepin), also known as 3'-deoxyadenosine (3'-Deoxyadenosine), is a nucleoside substance with antibacterial activity, which has a strong inhibitory effect on nuclear polyadenylate polymerase and can inhibit cancer. Cell growth, as well as anti-viral, anti-cancer and anti-fungal effects. In recent years, research at home and abroad has found that cordycepin contained in Cordyceps sinensis can effectively kill cancer cells, and regular consumption of Cordyceps sinensis can avoid and reduce the occurrence of cancer. Therefore, there is a strong demand in the international and domestic medical and health care raw material markets, and the active ingredient cordycepin has a huge potential. At present, the price of cordycepin is as high as tens of thousands of yuan per gram, so it is of...

Claims

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Application Information

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IPC IPC(8): C07H19/16A61K36/068
Inventor 唐庆九刘艳芳郝瑞霞张劲松杨焱
Owner SHANGHAI ACAD OF AGRI SCI
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