Corn fermentation partly substituted method for producing acetone-butanol

Abstract

The invention discloses a method partly replacing corn fermentation in acetone butanol production, including the following steps: raw material is prepared and boiled down for sterilization; when boiled mash is cooled down within a certain temperature range, strain is inoculated; after fermentation for a certain time, the mash is made into final acetone, butanol and ethanol products after filtration and distillation. The invention reduces the raw material cost in producing acetone butanol through adopting zymotechnics and eases the problem of high grain consumption; meanwhile, the invention increases the use value of B starch or B starch slurry in processors processing wheat flour, etc.

Description

technical field

[0001] The invention relates to a method for producing acetone-butanol by fermentation, in particular to a method for partially replacing corn to produce acetone-butanol by fermentation. Background technique

[0002] Among the many methods of producing acetone butanol, the production technology of acetone butanol by grain fermentation is relatively mature. It started large-scale industrialization during World War II and continued until the 1980s and 1990s. Replaced by the rising petrochemical technology. However, with the gradual depletion of petroleum resources and the increasingly severe ecological and environmental problems, countries around the world have begun to think about using renewable resources to replace petroleum resources and produce bulk chemicals through biotechnology to solve energy problems.

[0003] The existing fermentation production technology of acetone butanol is mainly based on corn fermentation, and some potatoes and sorghum grains ...

Examples

Embodiment 1

[0016] Fermentation of pure B starch slurry as raw material

[0017] Measure the initial total sugar content in B starch slurry, get B starch slurry 25ml in 250ml Erlenmeyer flask, add water 75ml, then add 6ml concentrated hydrochloric acid, plug the Erlenmeyer flask mouth with the stopper that has the plastic tube of 1 meter length, at 100 ℃ water bath in water bath for 45 minutes, take it out and cool, add 40% sodium hydroxide to adjust to slightly acidic, dilute the solution to 250ml, filter and measure the total reducing sugar in the hydrolyzate by Fehling’s method, and then calculate B The total sugar contained in the starch slurry is 10%.

[0018] Take 600ml of B starch slurry and add water to dilute it to 1000ml, the total sugar content after dilution is 6%, and then divide it into a 500ml Erlenmeyer flask with a liquid volume of 60%, and sterilize it in a sterilizing pot at 121°C for 1.5 Hour.

[0019] The medium was cooled to 37°C, and the EA2018 acetobutanol strain...

Embodiment 2

[0024] pure corn fermentation

[0025] Determination of the initial total sugar content in corn flour, weigh 2g of corn flour to a 250ml Erlenmeyer flask, add 100ml of water and then add 6ml of concentrated hydrochloric acid to hydrolyze it in a water bath for 45 minutes, then measure and calculate the total sugar content of corn flour by Fehling's method. About 70%, measuring method is the same as embodiment 1.

[0026] According to the sugar content of corn flour, take 85.7g of corn flour and add water to cook to control the volume after cooking to 1000ml. The initial total sugar of the medium is 6%.

[0027] The medium was cooled to 38°C, and the EA2018 acetobutanol strain in the test tube was inserted into the medium, and fermented in a 38°C incubator for 48 hours.

[0028] After the fermentation is finished, the fermented liquid is centrifuged at 12000 rpm for 5 minutes, and the contents of acetone, butanol and ethanol are measured by gas chromatography after taking it o...

Embodiment 3

[0032] B starch slurry replaces 80% corn flour with equivalent sugar content in the corn culture medium.

[0033] By calculation, measure 480ml of B starch slurry with 10% sugar content and 17g of corn flour and mix them for cooking, control the volume after cooking at 1000ml, divide the cooked mixture into 500ml Erlenmeyer flasks for sterilization, and the sterilization method is the same as Example 1.

[0034] The medium was cooled to 37°C, and the EA2018 acetobutanol strain in the test tube was inserted into the medium, and fermented in a 37°C incubator for 48 hours.

[0035] After the fermentation is finished, the fermented liquid is centrifuged at 12000 rpm for 5 minutes, and the contents of acetone, butanol and ethanol are measured by gas chromatography after taking it out. The solvent measurement results are shown in Table 3. After the fermentation, the residual sugar in the fermentation liquid is 2.5-3%. It can be seen that the culture medium obtained by such a mixing...

technical field

[0001] The invention relates to a method for producing acetone-butanol by fermentation, in particular to a method for partially replacing corn to produce acetone-butanol by fermentation. Background technique

[0002] Among the many methods of producing acetone butanol, the production technology of acetone butanol by grain fermentation is relatively mature. It started large-scale industrialization during World War II and continued until the 1980s and 1990s. Replaced by the rising petrochemical technology. However, with the gradual depletion of petroleum resources and the increasingly severe ecological and environmental problems, countries around the world have begun to think about using renewable resources to replace petroleum resources and produce bulk chemicals through biotechnology to solve energy problems.

[0003] The existing fermentation production technology of acetone butanol is mainly based on corn fermentation, and some potatoes and sorghum grains ...

Examples

Embodiment 1

[0016] Fermentation of pure B starch slurry as raw material

[0017] Measure the initial total sugar content in B starch slurry, get B starch slurry 25ml in 250ml Erlenmeyer flask, add water 75ml, then add 6ml concentrated hydrochloric acid, plug the Erlenmeyer flask mouth with the stopper that has the plastic tube of 1 meter length, at 100 ℃ water bath in water bath for 45 minutes, take it out and cool, add 40% sodium hydroxide to adjust to slightly acidic, dilute the solution to 250ml, filter and measure the total reducing sugar in the hydrolyzate by Fehling’s method, and then calculate B The total sugar contained in the starch slurry is 10%.

[0018] Take 600ml of B starch slurry and add water to dilute it to 1000ml, the total sugar content after dilution is 6%, and then divide it into a 500ml Erlenmeyer flask with a liquid volume of 60%, and sterilize it in a sterilizing pot at 121°C for 1.5 Hour.

[0019] The medium was cooled to 37°C, and the EA2018 acetobutanol strain...

Embodiment 2

[0024] pure corn fermentation

[0025] Determination of the initial total sugar content in corn flour, weigh 2g of corn flour to a 250ml Erlenmeyer flask, add 100ml of water and then add 6ml of concentrated hydrochloric acid to hydrolyze it in a water bath for 45 minutes, then measure and calculate the total sugar content of corn flour by Fehling's method. About 70%, measuring method is the same as embodiment 1.

[0026] According to the sugar content of corn flour, take 85.7g of corn flour and add water to cook to control the volume after cooking to 1000ml. The initial total sugar of the medium is 6%.

[0027] The medium was cooled to 38°C, and the EA2018 acetobutanol strain in the test tube was inserted into the medium, and fermented in a 38°C incubator for 48 hours.

[0028] After the fermentation is finished, the fermented liquid is centrifuged at 12000 rpm for 5 minutes, and the contents of acetone, butanol and ethanol are measured by gas chromatography after taking it o...

Embodiment 3

[0032] B starch slurry replaces 80% corn flour with equivalent sugar content in the corn culture medium.

[0033] By calculation, measure 480ml of B starch slurry with 10% sugar content and 17g of corn flour and mix them for cooking, control the volume after cooking at 1000ml, divide the cooked mixture into 500ml Erlenmeyer flasks for sterilization, and the sterilization method is the same as Example 1.

[0034] The medium was cooled to 37°C, and the EA2018 acetobutanol strain in the test tube was inserted into the medium, and fermented in a 37°C incubator for 48 hours.

[0035] After the fermentation is finished, the fermented liquid is centrifuged at 12000 rpm for 5 minutes, and the contents of acetone, butanol and ethanol are measured by gas chromatography after taking it out. The solvent measurement results are shown in Table 3. After the fermentation, the residual sugar in the fermentation liquid is 2.5-3%. It can be seen that the culture medium obtained by such a mixing...