Method for detecting food-borne enterocolitisyersinia genus by loop-mediated isothermal amplification

Abstract

The invention discloses a method for detecting foodborne Yersinia enterocolitica by using loop-mediated constant temperature amplification technology, which belongs to the technical field of bacterial detection. The constant temperature amplification technology is used for detection, and the detection method of the loop-mediated constant temperature amplification product includes: (1) 10,000 × g, 5 minutes, room temperature, positive visible white precipitate at the bottom of the test tube; (2) adding a chromogenic reagent, positive The result is green; (3) 1.5% agarose gel electrophoresis, the result is a ladder-like electrophoresis band; any one of the above three loop-mediated constant temperature amplification product detection methods can be selected for detection. This method has the characteristics of accurate detection, strong specificity and high sensitivity, can quickly and accurately identify specific target bacteria, improves detection efficiency, saves time, and thus solves the current situation that traditional detection methods cannot meet the increasing import and export goods.

Description

technical field [0001] The invention relates to a bacteria inspection technology, in particular to a method for detecting Yersinia enterocolitica, especially food-borne Yersinia enterocolitica, by using a loop-mediated constant temperature amplification reaction. Background technique [0002] Yersinia enterocolitica is a common bacterium in food, which can cause extremely serious food poisoning incidents and a variety of diseases, seriously threatening people's health. It is one of the microorganisms that must be inspected by customs in various countries. Rapid and accurate detection of Yersinia enterocolitica in food is a prerequisite for effective prevention and control of Yersinia infection. At present, the identification of bacteria in food is usually detected by traditional physiological and biochemical methods. Using traditional methods to detect Yersinia enterocolitica in food not only takes 4 days for colony culture, but also consumes a lot of manpower and labor. Ma...

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Problems solved by technology

[0005] In order to improve the efficiency of food detection, save time, and solve the problem that traditional detection methods cannot meet the growing status of import and export goods, the present invention has finally explored a method for the detection of enterocolitis using loop-mediated constant temperature amplification technology after countless experiments. The method of Yersinia, which has the characteristics of rapid detection, convenience and low cost

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