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Enterovirus immunofluorescence chromatographic assay test paper and its preparation method

A technology of enterovirus and detection test paper, which is applied in the field of immunofluorescence chromatography detection test paper, can solve the problems of prolonging the illness period, increasing patient pain and economic burden, and unclear diagnosis of pathogens, achieving objective results, quantitative detection, and results Objective and sensitive effects

Active Publication Date: 2011-08-10
BEIJING BOHUI INNOVATION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Diarrhea with dual or polyviral infections has been reported to be more severe, more rapidly progressive, and has a higher risk of death
[0007] Due to the lack of obvious specificity in the clinical manifestations of intestinal infectious diseases and the commonly used auxiliary examinations, there are risk factors for unclear diagnosis of pathogens in the early stage of the disease, which leads to the abuse of antibiotics in the early stage of the disease and the prolongation of the disease period, which increases the suffering of patients and the economy. objective factors of burden

Method used

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  • Enterovirus immunofluorescence chromatographic assay test paper and its preparation method
  • Enterovirus immunofluorescence chromatographic assay test paper and its preparation method
  • Enterovirus immunofluorescence chromatographic assay test paper and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 enterovirus four joint inspection test papers

[0041] Refer to attached figure 1 , the enterovirus multi-detection test paper of the present embodiment comprises base plate 1, the nitrocellulose that is embedded with quality control band (C band) and detection band (T1 band, T2 band, T3 band, T4 band) adhering to base plate Plain membrane 2, high-strength absorbent paper 3 covering one side of the nitrocellulose membrane, labeled antibody pad 4 covering the other side of the nitrocellulose membrane, and sample absorbent pad 5.

[0042] The C band region of the nitrocellulose membrane is embedded with goat anti-mouse IgG antibody, the T1 band is embedded with a monoclonal antibody against Norwalk virus, the T2 band is embedded with a monoclonal antibody against enteric adenovirus, and the T3 band is embedded with a monoclonal antibody against enteric adenovirus. Astrovirus-embedded monoclonal antibody, T4 with rotavirus-embedded monoclon...

Embodiment 2

[0099] 1. Specificity test of four joint inspection paper strips for enterovirus

[0100] Five specimens were selected (specimen 1 was a fecal specimen infected with Salmonella, specimen 2 was a fecal specimen infected with a single rotavirus, specimen 3 was a fecal specimen infected with a single enteric adenovirus, specimen 4 was a fecal specimen infected with a single astrovirus, and specimen 5 was a fecal specimen infected with a single astrovirus. A single norovirus-infected feces sample) was diluted 1:10 with sample diluent (0.5% Triton-X100+2% NaCl+0.1% Casein, 10mM pH7.2PB), and 50 μl was added to the four joint tests of enterovirus Put the sample pad on the paper strip, let it stand at room temperature for 15 minutes, and test the result with a fluorescence detector.

[0101]Table 1 Test values ​​of four joint test strips for enterovirus

[0102]

[0103] 2. Sensitivity test of four joint detection of enterovirus

[0104] Rotavirus antigen (concentration 3 μg / ml)...

Embodiment 3

[0109] Preparation of test strips for single detection of enterovirus

[0110] With reference to accompanying drawing 4, the enterovirus multi-detection test strip of the present embodiment comprises base plate 1, the nitrocellulose membrane 2 that is embedded with quality control band (C band) and detection band (T band) adhered to base plate , absorbent paper 3 covering one side of the nitrocellulose membrane, labeled antibody pad 4 and sample absorbent pad 5 covering the other side of the nitrocellulose membrane.

[0111] The C band region of the nitrocellulose membrane is embedded with goat anti-mouse IgG antibody, and the detection band is embedded with rotavirus monoclonal antibody or enteric adenovirus monoclonal antibody or astrovirus monoclonal antibody or Norwalk Viral monoclonal antibodies.

[0112] The pathogens to be tested are rotavirus or enteric adenovirus or astrovirus or norovirus.

[0113] 1. The monoclonal antibody of enterovirus labeled with fluorescein ...

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Abstract

The invention relates to a detecting testing paper for enterovirus fluorescence immune chromatographies, which comprises a base plate, a sampling absorbing mat, a marking antibody mat, a reaction film and a water absorbing mat. The sampling absorbing mat, the marking antibody mat, the reaction film and the water absorbing mat are connected closely in turn and are adhered on the base plate. The invention is characterized in that one or a plurality of enterovirus fluorescence marking monoclonal antibodies are wrapped by the marking antibody mat, one or a plurality of testing belts and a qualitycontrol belt are arranged on the reaction film, an enterovirus monoclonal antibody is fixed on each testing belt, and a sheep anti mouse IgC antibody is arranged on the quality control belt. Enteroviruses which are tested include one or a plurality of Norwalk virus, enteric adenovirus, human astrovirus and rotavirus. The purpose of qualitative or quantitative tests can be realized through a specific reaction of fluorescein or fluorescence microspheres marking antibody and applying special instrument tests.

Description

technical field [0001] The invention relates to an immunofluorescence chromatography detection test strip, in particular to an immunofluorescence chromatography detection strip for detecting common intestinal viruses, and detectable enteroviruses include Norwalk virus, intestinal adenovirus, Viruses, astroviruses and rotaviruses, the test strip can be used in related fields such as epidemiological monitoring and clinical testing. Background technique [0002] The immunochromatography method uses colloidal gold, fluorescein, enzymes, etc. as markers, and uses specific antigen-antibody reactions and the characteristics of the markers to conduct localization, qualitative and even quantitative research on antigens or antibody substances. Since Faulk and Taylor founded the colloidal gold labeling technology in 1971, Geoghegan_1 has applied the immunogold technology to detect the surface antigen of B lymphocytes, and established the immunogold technology (IGS) at the light microsc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/52
Inventor 杨奇周荣王长兵王海
Owner BEIJING BOHUI INNOVATION TECH