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Egg-derived bone-strengthening composition

A composition and protein hydrolyzate technology, applied in the direction of drug combination, medical raw materials derived from mammals, animal feed, etc.

Active Publication Date: 2012-03-28
PHARMA FOODS INT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these substances are drugs, and there is a risk of side effects such as tinnitus, headache, and loss of appetite, so special care must be taken when taking them, and they cannot be used as continuous food raw materials

Method used

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  • Egg-derived bone-strengthening composition
  • Egg-derived bone-strengthening composition
  • Egg-derived bone-strengthening composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 (manufacture of egg yolk protein hydrolyzate)

[0046] In 500kg of defatted egg yolk powder obtained by the same method as A, add 2.5 tons of water and Novozymes manufactured by Novozymes Investment Co., Ltd. Protease hydrolysis (trade name, protease derived from bacillus licheniformis) 25 kg, pH 7, 55 ° C for 3 hours to carry out the enzyme reaction, thereafter, at 80 ° C for 15 minutes to inactivate the enzyme, in Centrifuge at a centrifugal force of 3,000g for 20 minutes to remove the precipitate. After filtration, the filtrate is spray-dried to obtain about 140kg of egg yolk protein hydrolyzate.

[0047] The egg yolk protein hydrolyzate obtained in Example 1 was subjected to molecular weight analysis by gel permeation chromatography under the following conditions.

[0048] Column: Diol 60 (6.0×300 mm) (trade name, manufactured by YMC Co., Ltd.)

[0049] Eluent: 0.2M potassium phosphate buffer, 0.2M sodium chloride (pH6.9) / acetonitrile (70:30)

[0050] ...

Embodiment 2

[0067] Embodiment 2 (egg yolk protein hydrolyzate)

[0068] Add 3 L of water to 300 g of the defatted egg yolk powder obtained in A, and carry out the enzyme reaction under the enzyme conditions shown in Table 1. After that, after reaching 80°C in a boiling water bath, heat for 10 minutes to inactivate the enzyme, add 30 g and filter auxiliary agent (diatomaceous earth product), filtered with a Buchner funnel, and the obtained filtrate was freeze-dried to obtain 7 kinds of egg yolk protein hydrolyzates.

[0069] [Table 1]

[0070]

[0071] Note: "The area ratio of the specified molecular weight" refers to the area of ​​the portion with a molecular weight of 500 to 20,000 in the total area ratio of protein, peptide, and amino acid when the molecular weight distribution of egg yolk protein hydrolyzate is analyzed by gel permeation chromatography Compare.

[0072] Test 1 (osteoblast proliferation promoting activity)

[0073] Use α-MEM medium containing 10% FBS at 37°C, 5% ...

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Abstract

A bone-strengthening composition of the invention comprises as an active ingredient an egg-yolk protein hydrolysate in which a proportion of the area of the region of a molecular weight of 500 or more and 20,000 or less to the total proportion of the area of proteins, peptides and amino acids is 50% or higher in a molecular weight distribution analysis by gel filtration chromatography. The composition has an action of osteoblast growth promotion, bone growth and bone strengthening, is effective in the prevention and treatment of a variety of bone diseases such as osteoporosis, and is useful as a safe and inexpensive product that can be widely used for a food and drink, a pharmaceutical or a feed and can be taken daily and continuously. The egg-yolk protein hydrolysate is preferably a hydrolysate obtained by defatting egg yolk with an organic solvent and hydrolyzing the resulting powder with a protein hydrolyzing enzyme.

Description

technical field [0001] The present invention relates to a composition containing egg yolk protein hydrolyzate having bone growth and bone strengthening effects. In addition, the present invention also relates to food and beverages, medicines, feeds, etc., which contain the egg yolk protein hydrolyzate composition. The egg yolk protein hydrolyzate of the present invention has the effect of proliferating osteoblasts and promoting the proliferation of chondrocytes at bone growth ends. Therefore, it is possible to prevent or treat bone diseases caused by the disruption of bone metabolism balance, and it is also useful for bone strengthening in the growth period of children and other young generations. Background technique [0002] In recent years, the increase of various bone diseases such as the increase of osteoporosis and the increase of fracture rate in the younger generation is becoming a social problem. It is estimated that there are about 10 million patients with osteop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/305A23J3/34A23K1/16A23K1/165A61K35/54A61K38/00A61P19/00A61P43/00A23L15/00A23L33/10A61K38/02A61P19/10
CPCA23V2002/00A61K38/012A23L1/3053A23K1/1631A61K35/54A23J3/341A23K20/147A23L33/18A61P19/00A61P19/10A61P43/00A23V2200/306A23V2250/5428A47J37/1223A47J37/1266A47J37/1271A47J37/1285A47J37/129
Inventor 大井康之池旻映源伸介金武祚金惠京林康铉赵祥祐
Owner PHARMA FOODS INT CO LTD
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