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Method for producing ethanol and 2,3-butanediol by lignocellulose material

A lignocellulosic raw material, butanediol technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of low fermentation level, achieve the effect of reducing production costs and improving the utilization rate of raw materials

Inactive Publication Date: 2008-07-23
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Method 4 proposes to use wood hydrolyzate to produce 2,3-butanediol, but due to the limitation of process conditions, its fermentation level is very low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Production of ethanol and 2,3-butanediol from straw

[0036] 1. The stalk is cut, dedusted and crushed to 40 meshes, and added into a sulfuric acid solution with a concentration of 7wt%, and the weight ratio of the sulfuric acid solution to the plant fiber raw material is 8:1;

[0037] 2. Raise the temperature to 100°C and maintain this temperature for 2 hours. Filter the liquid-solid mixture obtained above, add CaO to the liquid phase until the pH value is equal to 8, stir, filter to remove the precipitate, add concentrated sulfuric acid to the filtrate to adjust the pH to 7, and use the filtrate to prepare seed culture medium and fermentation medium respectively according to Table 1. The reducing sugar content in the seed culture medium is controlled to be 20g / l, and the reducing sugar content in the fermentation medium is controlled to be 20g / l.

[0038] 3. A loop of Klebsiella pneumoniae (Klebsiella pneumoniae 10011, purchased from China Industrial Micro...

Embodiment 2

[0040] Example 2: Production of ethanol and 2,3-butanediol from corn cobs

[0041] 1. Corncobs are cut, dedusted, and crushed to 40 meshes, and added to a sulfuric acid solution with a concentration of 0.25%. The liquid-solid ratio of the sulfuric acid solution to the plant fiber raw material is 12:1;

[0042] 2. Raise the temperature to 200°C and maintain this temperature for 0.25 hours. Filter the liquid-solid mixture obtained above, add CaO to the liquid phase until the pH value is equal to 10, stir, filter to remove the precipitate, add concentrated sulfuric acid to the filtrate to adjust the pH to 7, and use the filtrate to prepare seed culture medium and fermentation medium respectively according to Table 1. The reducing sugar content in the seed culture medium is controlled to be 20g / l, and the reducing sugar content in the fermentation medium is controlled to be 40g / l;

[0043] 3. A ring of Klebsielia oxytoca (Klebsielia oxytoca 10370, China Agricultural Microorganism...

Embodiment 3

[0045] Example 3: Production of ethanol and 2,3-butanediol from bagasse

[0046] 1. Bagasse is cut, dedusted and pulverized to 40 mesh, adding sulfuric acid so that the acid concentration in the solution is 2%, and the liquid-solid ratio of the solution to the plant fiber raw material is 10:1;

[0047] 2. Raise the temperature to 160°C and maintain the temperature for 40 minutes. Filter the liquid-solid mixture obtained above, add CaO to the liquid phase until the pH value is equal to 9, stir, filter to remove the precipitate, add concentrated sulfuric acid to the filtrate to adjust the pH to 7, and use the filtrate to prepare seed culture medium and fermentation medium respectively according to Table 1. The reducing sugar content in the seed medium is controlled to be 20g / l, and the reducing sugar content in the fermentation medium is controlled to be 40g / l)

[0048] 3. A ring of Klebsiella pneumoniae (Klebsiella pneumoniae 10011, purchased from China Industrial Microorganis...

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PUM

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Abstract

The invention discloses a process preparation method for an ethanol and an 2,3-butanediol 2, 3 from a woody fiber material, belonging to the biochemical technical field, which is characterized in that a hemicelluloses inside the wood fiber material is hydrolyzed into a pentose, which is used to produce the 2,3-butanediol, the cellulose is enzymolysed into a hexose, which is used to produce the ethanol, and two high value added products ethanol and 2,3-butanediol are simultaneously got, and the concentration of the ethanol and the 2,3-butanediol in the fermentation liquid is increased by the means of filling more raw material. The process preparation method for an ethanol and an 2,3-butanediol 2, 3 from a woody fiber material has the advantages of effectively increasing the raw material utilization ratio, getting simultaneously the high concentration ethanol and 2,3-butanediol, and lowering the production cost, and having good industrial prospect.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular provides a method for simultaneously producing ethanol and 2,3-butanediol from lignocellulosic raw materials. Background technique [0002] Ethanol is widely used in beverage, food, flavor, condiment, medicine and energy industries, and occupies a relatively important position in the national economy. With the development of the national economy, the world's oil reserves have decreased sharply, environmental protection has been strengthened and vehicles are used With the increasing demand for fuel ethanol, the use of ethanol will be more extensive, the demand for ethanol will increase day by day, and the future of the ethanol industry will be brighter day by day. [0003] 2,3-butanediol (2,3-butanediol) is also an important chemical raw material. It is a colorless and odorless liquid that can be used as a fuel and can be used to prepare polymers, inks, perfumes, an...

Claims

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Application Information

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IPC IPC(8): C12P7/06C12P7/18C12R1/865C12R1/645
CPCY02E50/17Y02E50/10
Inventor 程可可张建安刘宏娟周玉杰
Owner TSINGHUA UNIV
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