Medical applications of stringy stonecrop ethamol soluble extractive

A technology for the alcohol extract of Cercis chinensis, which is applied in the field of medical application of the alcohol extract of C. cycle effect

Inactive Publication Date: 2008-07-30
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Weeping grass has been widely used clinically to treat sore throat, hot drench, water and fire burns, snake bites and various acute and chronic active hepatitis. Not yet reported

Method used

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  • Medical applications of stringy stonecrop ethamol soluble extractive
  • Medical applications of stringy stonecrop ethamol soluble extractive
  • Medical applications of stringy stonecrop ethamol soluble extractive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the preparation of weeping pot grass ethanol extract

[0018] Soak 500g of syringa hay in 5000mL of 95% ethanol for 5 days, filter with gauze, and collect the liquid medicine. Repeat 2 times, combine the liquid medicines, centrifuge to take the supernatant, evaporate the obtained supernatant under reduced pressure to remove the solvent, extract with 1000mL of petroleum ether to remove the lipid, evaporate the petroleum ether, then dissolve it with 100mL of methanol, and evaporate to dryness under reduced pressure. Dry to obtain alcohol extract.

Embodiment 2

[0019] Example 2: Inhibitory effect of ethanol extract on the proliferation of liver cancer cells

[0020] The ethanol extract was dissolved in DMSO and diluted with RPMI1640 medium to make the final concentration of DMSO 0.1%, and sterilized through a sterile filter for use. Human liver cancer cell line HepG2 was placed in RPMI1640 culture medium containing 10% calf serum at 37°C and 5% CO 2 cultured in an incubator. The tumor cells were cultured to the logarithmic growth phase, and the cell concentration was adjusted to 1×10 4 cells / ml, inoculated in 96-well plates, 100 μl / well, after 4 hours of adherence, added alcohol extracts to make the final concentrations of 25, 50, 100, and 200 μg / ml respectively, and set up a negative control group (RPMI1640 medium) And 0.1% DMSO solvent control group, set 6 parallel wells for each group, continue to culture for 24, 48, 72 hours, and detect the proliferation results by MTT method.

[0021] The effect of each dose group on HepG2 is...

Embodiment 3

[0025] Example 3: Cell cycle detection by flow cytometry:

[0026] 1×10 HepG2 cells were collected from the control group and the HepG2 cells after each dose of ethanol extract for 48 hours. 6 1, PI staining, cell cycle analysis by flow cytometry, the results are shown in Figure 1. Flow cytometry analysis showed that the ethanol extract could arrest liver cancer cells in G0 / G1 phase, thereby inhibiting its proliferative activity.

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Abstract

The invention relates to a new usage of sedum sarmentosun bunge extract, that is, an application of the sedum sarmentosun bunge in preparing anti-cancer medicine. The invention develops new medical usage of the extract of the sedum sarmentosun bunge and exploits a new application field. The extract of the sedum sarmentosun bunge has significant effect of inhibiting the proliferation of hepatoma cells, regulating and controlling cells period, and inhibiting relevant gene expression in RNA and protein level, so the invention can be used for preparing anti-tumor medicine.

Description

technical field [0001] The invention relates to a medical application of a Chinese herbal medicine extract, in particular to a medical application of an ethanol extract of Thuja chinensis. Background technique [0002] Sedum sarmentosum Bunge (Sedum sarmentosum Bunge), also known as Sedum sarmentosum, mouse tooth half branch, melon seed grass, Buddha nails, stone nails, and its use as medicine was first recorded in "Baicao Mirror". Department of Crassulaceae perennial succulent plants. Weeping grass is one of the commonly used traditional Chinese medicines recorded in the "Chinese Pharmacopoeia" in previous editions. Weeping grass is an important medicinal plant resource. It is harvested in summer and autumn to remove impurities. The fresh or dried whole herb is used as medicine. Treatment of jaundice, erysipelas ulcer, dysuria, carbuncle sores, acute and chronic hepatitis, and has good liver protection, immunosuppression, estrogen effect, angiotensin converting enzyme inh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/41A61P1/16A61P35/00
Inventor 张伟云黄丹丹邱水晴孙煜曹丽丽黄鹃陈维霞沈文斌
Owner NANJING UNIV
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