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Method for identifying blood albumen by chemically modified zinc oxide nanometer rod array film

A nanorod array and serum protein technology, which is applied in the field of using chemically modified ZnO nanorod array films to identify serum proteins, can solve problems such as increasing the signal-to-noise ratio, and achieve the effect of enhancing photoluminescence intensity and great practical significance.

Inactive Publication Date: 2008-08-06
SICHUAN UNIV
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Problems solved by technology

In these fluorescent bioassays, improving the detection sensitivity and increasing the signal-to-noise ratio are the biggest challenges currently facing

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  • Method for identifying blood albumen by chemically modified zinc oxide nanometer rod array film

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Embodiment Construction

[0012] The present invention will be described in detail below in conjunction with the examples and comparative examples, but the present invention is not limited to the scope of the examples.

[0013] Put the chemically modified ZnO array film into 0.08M EDC phosphate buffer solution (pH 8.0) and gently shake and soak at 8°C for 48h;

[0014] After taking out the base material and washing it five times with phosphate buffer solution, put it into 0.08mg / mL bovine serum albumin phosphate buffer solution (pH 8.0) and gently shake and soak at 8°C for 48h;

[0015] The base material was taken out again and washed five times with phosphate buffer solution, and then dried with warm air to obtain serum protein grafted ZnO nanorod array film;

[0016] This bioarray film was analyzed with a fluorescence spectrophotometer, and serum proteins were identified by the intensity of the excitation light.

[0017] Compared with the existing fluorescence detection technology, the present inven...

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Abstract

The invention discloses a serum protein identification method through chemical modified ZnO nanometer rod array thin film. The invention adopts a fluorescence spectrometer to detect the array thin film and determines the serum protein through luminous intensity of the biologically connected nanometer rod array. The invention has the following steps in order: firstly connecting a polypeptide condensing agent to the surface of chemically modified ZnO nanometer rod array thin film; then connecting the serum protein to the ZnO nanometer rod array thin film; finally analyzing the biological array thin film with the fluorescence spectrometer, and identifying the serum protein through the detected luminous intensity. The invention adopts the chemical modification method to graft the serum protein onto the surface of the ZnO nanometer rod array to form the biological array thin film, and adopts the fluorescence spectrometer to carryout biological signal detection to identify the serum protein, thereby enabling the ZnO nanometer rod to have biological detection practical significance. The invention has wide application value in the biological detection and the disease diagnosis fields, etc.

Description

technical field [0001] The invention relates to a method for fluorescent detection and identification of serum proteins, in particular to a method for identifying serum proteins by chemically modifying ZnO nanorod array films. Background technique [0002] Zinc oxide (ZnO) is zinc oxide as an important semiconductor and piezoelectric nanomaterials. The bandgap width and exciton binding energy are 3.37eV and 60meV, respectively. Efficient exciton luminescence can be obtained at room temperature. Display, electroluminescence, low-voltage field emission display, laser, optoelectronic devices, sensors and energy converters have broad application prospects. Compared with other semiconductor nanomaterials, nano-zinc oxide has exceptionally rich special morphology, such as: flower shape, ring shape, ribbon shape, tetrapod shape, sheet, tower shape, cage shape and propeller shape, etc. These rich and special morphologies may enable them to have some new properties and are expected ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/64
Inventor 黄忠兵尹光福姚亚东廖晓明康云清
Owner SICHUAN UNIV
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