Active component in albizia julibrissin with inhibiting angiogenesis function and preparation method and application thereof

Abstract

The invention relates to an active ingredient which has role in inhibiting angiogenesis in cortex albiziae. The active ingredient is a saponin compound which has applications of treatment and prevention of diseases about angiogenesis and anti-tumor angiogenesis, can be used in medicines which can prevents angiogenesis, or can be used as conventional medicine carrier and / or excipient, or is applied in preparation of health-care food or cosmetics for prevention of angiogenesis, so that the invention also provides the preparation method of the active ingredient, which is that the dry tree barks, stem skins, leaves, flowers, seeds, pods, or roots of the cortex albiziae are extracted by organic solvents, organic solvents with water or water; the extracting solution is filtered and condensed into extract; the extract is eluted and separated by a D-101 shape macroporous resin, then the effective groups are collected and are eluted and separated by a silica gel chromatography column, another effective groups are collected and separated by an RP-styrene column so that an effective group is obtained, the solvent is recycled to be an extract which is freezed, dried and then made into drymeal.

Description

(1) Technical field [0001] The invention relates to the active ingredient in Albizia Julibrissin bark which has the effect of inhibiting angiogenesis, and also relates to the preparation method and application of the active ingredient. (2) Background technology [0002] Angiogenesis is the process by which tissues utilize existing blood vessels to create new blood vessels. The vascular endothelial cells of normal adults are generally in a relatively quiescent state. Under physiological conditions, the endothelial cells divide once every few years on average, and only occur in the ovary and endometrium during trauma repair or menstrual cycle. However, many diseases such as tumors can occur in tissues The new vascular endothelial cells of the new blood vessels proliferate rapidly, and their proliferation cycle is equivalent to the germinal cells of bone marrow or intestinal epithelial cells [1, Tímár J, Dme B, Fazekas K, Janovics A, Paku S. Angiogenesis-dependent diseases and...

AI Technical Summary

Problems solved by technology

Albizia Julibrissin extract has a wide range of in vivo and in vitro anti-tumor cell proliferation activities, but its anti-cancer mechanism is still unclear, which limits its clinical application of anti-cancer effects [13, Tsuyoshi Ikeda, Tetsuya Kajimoto, Toshihiro Nohara, Jun- ei Kinjo, Chi-Huey Wong. Preparation of a neoglycolipid carrying the oligosaccharide component of saponin from Albizzia julibrissin. Tetrahedron Letters. 1995, 36(9): 1509-1510.14, Kun Zou, Yuying Zhao, Guangzhong Tu, Jingrong Rui, Zhongyhua Jia Zhang. Two diastereomeric saponins with cytotoxic activity from Albizia julibrissin. Carbohydrate Research. 2000, 324(3): 182-188.15, Kun Zou, Yuying Zhao, Guangzhong Tu, Junhua Zheng, Ruyi Zhang. A New Isomer of Julibroside J 2 from Albizia julibrissin.J Asian Natural Products Research.1998, 1(1):59-66.16, Zou Kun, Zhao Yuying, Li Deyu, Xu Feng, Zheng Junhua, Zhang Ruyi. Fat-soluble components of Albizia julibrissin. Journal of Beijing Medical University, 1999, 31(1):32-34.]

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