Method for establishing around vascellum endometrial hyperplasia animal model

A vascular intima, animal model technology, applied in the field of zoology, achieves important practical value and theoretical research value, good repeatability, and obvious clinical symptoms.

Inactive Publication Date: 2008-08-20
SHANDONG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Vascular intimal hyperplasia has tortured patients for a long time, and also brought a heavy burden to families and society

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1.1 To establish a rabbit animal model of peripheral vascular intimal hyperplasia (Intima hyperplasia, IH), the steps are:

[0026] (1). Select 10 male healthy New Zealand white rabbits weighing 1.2-1.8kg, ordinary grade, provided by the Rabbit Breeding Factory of Shandong Academy of Agricultural Sciences, production license number "SCXK (Lu) 20040013", and randomly divided into control group and the test group, each with 5 rats. Oridonin A for injection was injected into the experimental group by auricular vein injection once a day at a dose of 7.2mg / kg·d -1 , continuous injection for 10 days; the control group was fed normally for 10 days. The experimental group and the control group maintained the same feeding conditions and feeding environment.

[0027] (2). The animals in the experimental group and the control group were sacrificed, and the auricular artery was isolated, fixed in situ with 10% formalin solution, dehydrated, embedded, sectioned, and stained with V...

Embodiment 2

[0040] 2.1 To establish a rat animal model of intima hyperplasia (IH), the steps are as follows:

[0041] (1). Take 10 healthy male SD rats, weighing 310-350g, provided by the Experimental Animal Center of Shandong University, production license number "SCXK (Lu) 20040004", and randomly divide them into control group and test group (five rats each) ). Oridonin for injection was injected into the experimental group by tail vein injection once a day at a dose of 12mg / kg·d -1 , continuous injection for 10 days; the control group was fed normally for 10 days. The experimental group and the control group maintained the same feeding conditions and feeding environment.

[0042](2). SD rats were killed, and blood vessels of 0.5 cm were taken from the same position of the peripheral arterioles of each rat, fixed in formalin, dehydrated, embedded in paraffin, and sliced ​​8 to 10 pieces evenly in each section of blood vessels. For HE staining, 6 slices were randomly selected in each ...

Embodiment 3

[0054] 3.1 To establish a canine animal model of intimal hyperplasia in peripheral blood vessels, the steps are as follows:

[0055] (1). Take 10 healthy Beagle dogs, male or female, weighing 15-20kg. They were raised routinely and provided by the Experimental Animal Center of Shandong University. The production license number is "SCXK (Lu) 20040004". Group (5 each). For the test group, subcutaneous cephalic vein injection of oridonin for injection was performed once a day at a dose of 4.2 mg / kg·d -1 , continuous injection for 10 days; the control group was fed normally for 10 days. The experimental group and the control group maintained the same feeding conditions and feeding environment.

[0056] (2). All the animals in the two groups were killed, and 0.5 cm blood vessels were taken from the same part of the peripheral arterioles, and the fixed peripheral arterioles were embedded in paraffin, sliced ​​serially at a thickness of 5 μm, stained with hematoxylin-eosin (HE), an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for building peripheral vascular intima hyperplasia animal model, which comprises the following steps: intravenous injection is conducted upon a healthy mammal using oridonin; 7 to 12 days of continuous injection should be made with once of injection for each day; dosage is 0.30 mg / kg.d <-1> to 15 mg / kg.d <-1>; then the animal is killed; ear artery or peripheral small artery of the animal is taken down and used as pathological section; under light microscope, intima is obviously thickened; vascular cavity area is obviously narrowed; significant difference exists for increased thickness of intima and vascular cavity area compared with pathological section of control group, peripheral vascular intima hyperplasia animal model has been successfully built. The method has the advantages of good repeatability, obvious clinical symptoms, simple, safe and effective operation method, important practical value and theoretical study value. The animal model building rate can reach 100% and can be used for large-scale animal experiment.

Description

technical field [0001] The invention relates to a method for establishing an animal model of peripheral vascular intimal hyperplasia, belonging to the technical field of zoology. Background technique [0002] Animal disease models are mainly used in the research of experimental physiology, experimental pathology and experimental therapeutics (including new drug screening). The development of human diseases is very complicated. If people themselves are used as experimental objects to deeply explore the mechanism of disease occurrence, the development of medicine will be very slow, and the accumulated clinical experience is limited in time and space, and many experiments are in Moral and methodological constraints are also imposed; and indirect research with the help of animal models can consciously change those factors that are impossible or difficult to exclude under natural conditions, so as to more accurately observe the experimental results of the model and conduct compar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61B19/00A61B90/00
Inventor 厉保秋久田黄山英
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap