Salviol acid B phosphatide complexes and method of preparing the same
A technology of phospholipid complex and salvianolic acid, which is applied in the field of medicine, can solve the problems of low bioavailability of salvianolic acid B, and achieve the effect of improving absorption
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Embodiment 1
[0026]Take 30.2g of salvianolic acid B, add 60.4g of soybean lecithin, dissolve in 1000ml of ethyl acetate, stir at 30°C for 2 hours to obtain a clear brown solution, remove ethyl acetate under reduced pressure at 40°C, add 500ml of dichloromethane to redissolve , filtered to remove the precipitate, and then evaporated under reduced pressure to remove dichloromethane to obtain about 90 g of brown-yellow dry matter, which is the salvianolic acid B phospholipid complex.
[0027] Adopt mobile phase condition to be acetonitrile: methanol: 0.1% phosphoric acid=25: 10: 65 carry out HPLC analysis to salvianolic acid B, phospholipid, salvianolic acid B phospholipid complex and mixture, its result is as attached figure 1 shown. The results showed that the chromatographic behavior of salvianolic acid B and its phospholipid complex was consistent under this condition, and the retention time was 9.262min and 9.113min, which indicated that the formation of salvianolic acid B and phospholip...
Embodiment 2
[0033] Take 5.5g of salvianolic acid B, add 5.5g of soybean lecithin, dissolve together in 1000ml of acetone, stir at 30°C for 1 hour to obtain a clear brown solution, remove the acetone under reduced pressure at 30°C, add 500ml of dichloromethane to redissolve, filter to remove After the precipitation, dichloromethane was evaporated under reduced pressure to obtain about 11 g of a brownish-yellow dry product, which was the salvianolic acid B phospholipid complex.
Embodiment 3
[0035] Take 30g of salvianolic acid B, add 120g of soybean lecithin, dissolve together in 600ml of chloroform, stir at 30°C for 4 hours to obtain a clear brown solution, remove chloroform under reduced pressure at 35°C, add 500ml of dichloromethane to redissolve, filter to remove the precipitate, and then Dichloromethane was removed by evaporation under reduced pressure to obtain about 150 g of a brownish-yellow dry product, which was the salvianolic acid B phospholipid complex.
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