Mucor rouxii and method for producing xylanase by using the same

A technology for xylanase and mucor, which is applied in the field of Mucor XJYL HN0512 and its induced production of xylanase, which can solve the problem that xylanase does not have high temperature resistance and strong alkali resistance, and is not suitable for xylanase. and other problems, to achieve the effect of wide pH adaptation range, high thermal stability and broad application prospects

Inactive Publication Date: 2008-09-10
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the produced xylanase does not have the ability to withstand high temperature and strong alkali
Its disadvantage is that the produced xylanase is not suitable for action under acidic conditions

Method used

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  • Mucor rouxii and method for producing xylanase by using the same
  • Mucor rouxii and method for producing xylanase by using the same
  • Mucor rouxii and method for producing xylanase by using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Fermentation medium (100ml): xylan 1.0g, yeast powder 1.0g, peptone 1.0g, FeSO 4 0.03g, CaCl 2 0.1g, Tween80 0.05ml, MgSO 4 ·7H 2 O 0.03g, Vitamin B 1 0.003g, CaCO 3 Adjust the pH to be 7.5, then insert 80-100 mg of Mucor rousei HN0512 bacterial strain preserved on the PDA slant, culture on a shaking table (150 rpm / min), cultivate xylanase for 72 hours at 28 ° C, and detect xylanase activity as 337.44U / L.

Embodiment 2

[0048] Fermentation medium (100ml): xylan 1.5g, yeast powder 0.5g, peptone 1.0g, FeSO 4 0.03g, CaCl 2 0.1g, Tween80 0.05ml, MgSO 4 ·7H 2 O 0.03g, Vitamin B 1 0.003g, NaOH to adjust the pH to 6.5, then insert 80-100mg of Mucoralus rugerii HN0512 strain preserved on a PDA slant, culture on a shaking table (180rpm / min), cultivate at 25°C for 72 hours to obtain xylanase, and detect xylanase Carbohydrase activity was 293.08U / L.

Embodiment 3

[0050] Fermentation medium (100ml): xylan 1.5g, yeast powder 1.3g, peptone 0.5g, FeSO 4 0.04g, CaCl 2 0.1g, Tween80 0.05ml, MgSO 4 ·7H 2 O 0.03g, Vitamin B 1 0.003g, NaOH to adjust the pH to 7.0, then insert 80-100mg of Mucoralus rugerii HN0512 strain preserved on a PDA slant, cultivate on a shaking table (150rpm / min), cultivate at 25°C for 72 hours to obtain xylanase, and detect xylanase Carbohydrase activity was 231.54U / L.

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Abstract

The invention discloses a Mucor rouxianus XJYL HN0512 and a method for fermentation production of xylanase by adoption of the strain, wherein, the strain is preserved in China Center for Type Culture Collection; the preservation time is January 18th, 2008 and the preservation No. is CCTCC NO:M 208014. The method is as follows: the Mucor rouxianus HN0512 strain is inoculated into a fermentation medium which contains xylans, yeast powder, peptones and other nutritive materials for fermentation culture, and the xylanase is produced through induction and fermentation under aerobic conditions. The xylanase produced has wide pH adaptability and high temperature tolerance and has wide application prospect in aspects of foodstuffs, feedstuffs, paper making, energy and the textile industry.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to Mucor rousei XJYL HN0512 and a method for inducing xylanase production thereof. Background technique [0002] Xylanase (E.C 3.2.1.8) is a class of enzymes that degrade β-1,4-xylosidic bonds in xylan molecules in an endo-cutting manner, and can hydrolyze xylan into xylooligosaccharides and xylose. These xylo-oligosaccharides have the effect of significantly proliferating bifidobacteria in the intestinal tract, can improve the immunity of humans and livestock, and reduce various diseases; as a growth stimulator and growth promoter for crops, increase crop yield; increase pulp yield, Improve paper quality. Therefore, there are huge potentials and broad application prospects in food, feed, papermaking, energy, and textile industries. [0003] Xylanase is widely distributed in nature and exists in marine and terrestrial bacteria, marine algae, fungi and rumen of ruminants, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/42C12R1/785
Inventor 姚冬生邢克克吴葵刘大岭谢春芳梁郁强
Owner JINAN UNIVERSITY
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