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Solid fermentation method for producing tannase by using gallnut raw material

A solid fermentation, gallnut technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low tannase efficiency, hinder the application of tannase, and high market price, and achieve less sterilization. The effect of bacteria technology, simplifying equipment and production process, and reducing production cost

Inactive Publication Date: 2008-09-10
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solid fermentation method has incomparable advantages over the liquid fermentation method, but the efficiency of solid fermentation to produce tannase is still not high, its general enzyme activity is only 14U / g dry weight, and the market price is expensive, which hinders the application of tannase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Inducer gallnut powder is crushed by a grinder and passed through a 40-mesh sieve. Take by weighing the mixture of 5g gallnut powder and bran and place in the 250ml triangular flask, gallnut powder content is 5 parts in the mixture, then adds inorganic salt solution (1 part of NH 4 NO 3 +0.1 part of MgSO 4 ·7H 2 (0+0.1 part of NaCl) 5ml, make fermentation substrate mix homogeneously, directly inoculate 10 parts of aspergillus niger spore suspensions by weight without autoclaving, the concentration of aspergillus niger spore suspensions is 1.0 * 10 9 ~10×10 9 cells / ml, cultured statically at 30°C for 96 hours. Take out the Erlenmeyer flask that has been cultivated for 96 hours, add 50ml pH=5.0 citric acid buffer solution in the Erlenmeyer flask, shake and extract at 180r / min for 1h, filter with qualitative filter paper to obtain the crude enzyme liquid of tannase, the activity of tannase can be It is as high as 38.3U / g dry weight, which is 2.74 times of t...

Embodiment 2

[0018] Example 2: Inducer gallnut powder is crushed by a grinder and passed through a 40-mesh sieve. Take by weighing the mixture of 5g gallnut powder and bran and place in the 250ml conical flask, gallnut powder content is 20 parts in the mixture, then add inorganic salt solution (1 part of NH 4 NO 3 +0.1 part of MgSO 4 ·7H 2 (0+0.1 part of NaCl) 5ml, the fermentation substrate is mixed homogeneously, the pH value 6 that adds acid solution or adds lye to regulate culture medium, directly inoculate 5 parts of Aspergillus niger spore suspensions by weight without autoclaving solution, the concentration of Aspergillus niger spore suspension was 1.0×10 9 ~10×10 9 cells / ml, cultured statically at 30°C for 120 hours. Take out the Erlenmeyer flask that has been cultivated for 120 hours, add 50ml of pH in the Erlenmeyer flask and be 5.0 citric acid buffer solution, 180r / min vibration leach for 1h, filter with qualitative filter paper to get tannase crude enzyme liquid, the activ...

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PUM

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Abstract

The invention discloses a method for solid fermentation to produce tannins by utilization of gallnut uncooked materials, wherein, mixture of gallnut powder and bran with a weight ratio between 5:95 and 35:65 is taken as a culture medium; inorganic salt solution the weight of which is 0.6 to 2.1 times of that of the mixture is added into the mixture and uniformly mixed; the uncooked materials are directly inoculated into aspergillus niger spore suspension; after stand culture for 28 to 168 hours at the temperature of 28 to 35 DEG C, and tannin crude enzymes are obtained through extraction by utilization of citrate buffer. The method takes the gallnut powder and the bran as raw materials, wherein, the gallnut powder is an inducer for production of the tannins. The method adopts the uncooked materials to be directly inoculated into aspergillus niger solids for fermentation production of the tannins without high-pressure steam sterilization treatment according to the characteristic that Chinese specialty gallnuts are easy to be burned through high-pressure steam sterilization treatment. The method avoids damage of high temperature on the raw materials, simplifies equipment and the production flow and improves the production efficiency.

Description

technical field [0001] The invention relates to a production method of tannase, in particular to a method for producing tannase by solid fermentation of gallnut raw meal. Background technique [0002] Tannase (Tannase EC1.1.20), also known as tannin ester acyl hydrolase, can hydrolyze the ester bond and depside carboxyl bond in gallic tannin to generate gallic acid and glucose. Tannase is an inducible enzyme, which can be synthesized by some microorganisms in the presence of inducers such as tannic acid. Aspergillus niger and Aspergillus oryzae are typical representatives. Tannase is widely distributed and has many uses. The U.S. Food and Drug Administration (FDA) has identified tannase as a safe product, and tannase has also been approved for use in the food industry in Japan. But at present, the production efficiency of tannase is low, the market price is high, and the application in various industries is limited. Chinese specialty gallnut is an inducer for the producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16C12R1/685
Inventor 邱树毅王广莉保玉心李秧针吴远根
Owner GUIZHOU UNIV
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