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Method for separating and purifying mitochondrion from pear pollen tube

A technology of separation and purification, pollen tube, applied in the direction of plant cells, etc., to achieve the effect of strong scientific, solid theoretical basis, and simple method

Inactive Publication Date: 2008-09-17
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
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Problems solved by technology

So far, there is no report on the isolation and purification of complete and active mitochondria from pear pollen tubes in the world

Method used

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  • Method for separating and purifying mitochondrion from pear pollen tube
  • Method for separating and purifying mitochondrion from pear pollen tube
  • Method for separating and purifying mitochondrion from pear pollen tube

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Embodiment Construction

[0029] As we all know, the isolation and purification of pollen tube mitochondria is the premise of conducting experimental research on mitochondrial proteomics and genomics. So far, due to the low extraction efficiency, it often takes tens of grams to With several kilograms of plant material, the original method cannot meet the needs of isolating and purifying mitochondria from pollen tubes with small volume and light weight. At present, there is no report on the isolation and purification of mitochondria from pollen tubes of Rosaceae plants in the world. We applied the method of the present invention to obtain complete and active pear pollen tube mitochondria. Using the method of western blotting, the mitochondrial components successfully detected positive bands of unique proteins in the mitochondria. Foundations in Mitochondrial Proteomics and Genomics. This technology of isolating and purifying mitochondria from micropollen tubes will be of great significance for the stud...

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Abstract

The invention relates to a method for separating and purifying mitochondria in a pear pollen tube, and belongs to the biotechnology field. The method comprises the steps of collecting pollen, culturing, preparing mitochondria extracting solution, preparing cleaning solution, preparing Percoll gradient liquid, the course of separating pollen tube mitochondria, filtering the cultured pollen tube by a cell screen, adding the mitochondria extracting solution to pulverize, obtaining cytoplasm liquid, purifying and cleaning with the Percoll gradient liquid to obtain the mitochondria after oscillation and centrifugation. Through the method, complete active pollen tube mitochondria is obtained, and the mitochondria separating and purifying efficiency is improved. The technology for separating and purifying mitochondria from the trace pollen tube which can be used as experimental material for pollen tube physiology and biochemistry and cell heredity has a significant meaning on researches and developments of the quick growth of the pollen tube, and the growth inhibiting technology for incompatible pollen tube.

Description

technical field [0001] The invention relates to a method for separating and purifying mitochondria from pear pollen tubes, relates to plant tissue cell homogenate and obtaining complete and active mitochondria, and belongs to the field of biotechnology. Background technique [0002] Mitochondria are the main organelles of cells, the "power factory" of cells, the main place for respiratory metabolism, and the center of energy metabolism and material conversion in cells, playing a key role in maintaining cell dynamic balance; and mitochondria contain their own genome The structure, capable of self-replication, can encode some important enzyme components in the process of respiratory metabolism, and is a relatively independent genetic system outside the nucleus; in addition, mitochondria are also directly related to the programmed death of animal and plant cells. [0003] The study of mitochondrial proteome and genome is an important means to understand various physiological an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
Inventor 张绍铃王春雷陈功吴俊吴华清
Owner NANJING AGRICULTURAL UNIVERSITY
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