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HIV antibody immune magnetic beads rapid detection reagent kit

A technology for human immunodeficiency and detection kits, which is applied in measurement devices, instruments, scientific instruments, etc., to avoid human errors, easy to operate, and accurate to determine results.

Active Publication Date: 2013-05-29
天津中新科炬生物制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the development of rapid diagnostic reagents for HIV antibody detection using nano-immunomagnetic beads

Method used

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  • HIV antibody immune magnetic beads rapid detection reagent kit
  • HIV antibody immune magnetic beads rapid detection reagent kit
  • HIV antibody immune magnetic beads rapid detection reagent kit

Examples

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Embodiment 1

[0039] Embodiment 1: Preparation of human immunodeficiency virus (HIV) antibody nano-immunomagnetic bead rapid detection reagent strip:

[0040] 1 main material

[0041] 1.1 Recombinant antigens: HIV gp41, gp36: products of Biotech Atlantic Inc (BAI.) of the United States, used for coating and labeling of reagents; nano-immune magnetic beads: products of AdemTech, France, with a diameter of 200nm and carboxyl modification on the surface; NC membrane: Millipore Company products; EDC, MES, BSA: Sigma products. Other commonly used reagents are analytical reagents.

[0042] 1.2 HIV antibody national reference product (colloidal gold): developed by China Institute for the Control of Pharmaceutical and Biological Products. Including 20 positive sera, 20 negative sera, 3 sensitivity sera, and 1 precision sera.

[0043] 1.3 Serum containing interfering substances: including 10 serums of common interfering substances hyperlipidemia, hemolysis, and jaundice, 10 serums containing posi...

Embodiment 2

[0061] Embodiment 2: Preparation of human immunodeficiency virus (HIV) antibody nano-immune magnetic bead rapid detection kit 10:

[0062] figure 2 Front view of the lid of the immunomagnetic bead detection reagent plastic box. image 3 Rear view of the lid of the immunomagnetic bead detection reagent plastic box. Figure 4 The bottom front view of the immunomagnetic bead detection reagent plastic box. Such as figure 2 , image 3 , Figure 4 As shown, the human immunodeficiency virus (HIV) antibody nano-immune magnetic bead rapid detection kit 10 is composed of a lid 11, a box bottom 20, a human immunodeficiency virus (HIV) antibody nano-immune magnetic bead rapid detection reagent strip, and the lid 11. The bottom of the box 20 is integrally formed by plastic injection molding. The box cover 11 and the bottom of the box 20 are both arc-shaped elongated upper corners and an upward square depression is set in the middle of the lower side.

[0063] The length and width o...

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Abstract

The invention relates to a quick detection kit for detecting human immunologic deficiency disease (HIV) using nm immunomagnetic beads, related to biology applied technique field. The detected sample is added on the sampling pad of the test paper from the sampling hole and the sample dissolves the mark beads and chromatography is performed on the NC membrane, and then the data on the test paper isdetected using magnetic force detector in preset time and the result is decided based on the read data. The kit is used for screening or clinical diagnosis of the HIV infection with high sensitivity,specificity, quickspeed, convenience and suitable for detection on site and real time recording and storing the result.

Description

technical field [0001] The invention relates to the technical field of biological applications, and relates to HIV antibody diagnostic reagents, in particular to an immunochromatography rapid diagnostic reagent for detecting HIV antibody with nanometer immunomagnetic bead technology. Background technique [0002] Acquired Immunodeficiency Syndrome (AIDS), referred to as AIDS, is a serious infectious disease caused by Human Immunodeficiency Virus (Human Immunodeficiency Virus, HIV) infection. Since the first AIDS patient was diagnosed in the United States in 1981, according to the latest WHO data, by the end of 2007, there were estimated to be 33 million HIV-infected people in the world, and there were about 700,000 HIV-infected people in China. [0003] HIV transmission routes mainly include blood transmission, sexual contact, drug injection and mother-to-child transmission. The average incubation period in the human body is 7 to 10 years. Patients in the latent period of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/532
Inventor 李洲杨发青
Owner 天津中新科炬生物制药股份有限公司
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