Phlegm solution process reagent with Chinese herbal medicine aqueous extract as principal ingredient

Abstract

The invention belongs to medical field. The invention creatively sues the natural ingredient of the Chinese medicinal herb as the main ingredient of the outer processing reagent of phlegm liquor. Specifically the method processing water extraction after mixing the purpura and bambusae with a certain proportion, taking the water extracting liquor as the main ingredient to process the phlegm liquor, cooperating to the neutral formalin nitrate buffer solution or the isotonic nitrate buffer solution to fix and protect the cast-off cells of the phlegm liquor sample. The reagent can process the phlegm liquor in static state without any assistant ways such as heating, blending; the assistant instrument is centrifuger; there is no special training request for the user. The invention can be used in the temperature range of 4 to 45 deg C., further stored and transported in the temperature range -18 to 50 deg C.; the cost is low and can be applied to large range lung cancer census.

Description

technical field [0001] The invention relates to a reagent which takes Chinese herbal medicinal water extract as the main component and can treat sputum in vitro under static conditions, is suitable for exfoliative cytology diagnosis and large-scale lung cancer general survey, and belongs to the medical field. Background technique [0002] The method of smear microscopy using exfoliated cells in trachea and bronchial secretions (sputum) has always been the main method for lung cancer screening. This method is widely used in lung cancer screening because it has no damage or little damage. [0003] The current clinical detection method is to take a part of the sputum sample for direct smear microscopy. Because the sputum sample has a lot of viscous substances, poor fluidity and low cell content, it is impossible to effectively collect the sample cells on the glass slide, and there are a large number of mucus-covered cells on the smear, which can easily lead to missed diagnosis ...

AI Technical Summary

Problems solved by technology

[0006] 1) Enzymatic method: not only the operation is cumbersome due to the need for auxiliary conditions such as constant temperature and stirring, but also the difficulty of storage and transportation is increased because it is sensitive to temperature. At the same time, the high cost of reagents also makes it difficult to carry out census applications;

[0007] 2) strong alkali treatment method, mechanical stirring method, etc.: there are also deficiencies such as requiring specific temperature conditions or cumbersome operations;

[0008] 3) In the Chinese Patent Publication No. CN1168470A, the sputum is collected in normal saline and then centrifuged at 4° C. at 40,000 g: this method not only reduces the processing efficiency because there is no active ingredient for directly treating sputum in the reagent, but also can Centrifuges up to 40,000g are not usually equipped in clinical units, not to mention the temperature requirement of 4°C, so this method is not suitable for exfoliative cytology diagnosis and large-scale lung cancer screening;

[0009] 4) In the Chinese Patent Publication No. CN1694726A, "natural thioredoxin", "protein or peptide containing reduced thioredoxin" or "nucleic acid molecule encoding thioredoxin" is used to treat sputum: The raw materials used in this method need to be extracted from specific microorganisms or plants to make finished products, which will inevitably increase the overall cost and complicate the production process. conditions are required

Similar components such as cysteine ​​and its hydrochloride are not suitable as in vitro treatment reagents for exfoliative cytology diagnosis and large-scale lung cancer screening