Special micro-flow control chip and single sampling continuous separation electrophoresis method

A microfluidic chip and electrophoresis technology, applied in separation methods, filtration separation, semi-permeable membrane separation, etc., can solve problems such as buffer leakage, poor stability, and large fluctuation range of sample peak height, so as to avoid dilution and enhance repetition performance, and the effect of improving accuracy

Inactive Publication Date: 2008-10-01
DALIAN UNIV OF TECH
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Problems solved by technology

[0003] However, when the continuous electrophoresis separation experiment is carried out on the microfluidic chip with the currently commonly used entrainment sampling method, the buffer solution will leak, and the buffer solution will enter the sample solution pool through the sampling channel, resulting in the dilution of the sample solution and resultin

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  • Special micro-flow control chip and single sampling continuous separation electrophoresis method
  • Special micro-flow control chip and single sampling continuous separation electrophoresis method
  • Special micro-flow control chip and single sampling continuous separation electrophoresis method

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[0014] The specific embodiments of the present invention will be described in detail below in conjunction with the technical solution and the drawings.

[0015] Such as figure 1 As shown, the dedicated microfluidic chip is based on the traditional micromachining method. The chip is composed of an upper cover sheet 9 and a lower substrate 8. On the upper cover sheet 9, a sample liquid pool 1 with a diameter of 3 mm is distributed. Cell 2, sample waste liquid pool 3, buffer waste liquid pool 4, shunt storage pool 5; multiple micro channels are distributed on the lower substrate 8, and the cross area is double T-shaped or cross-shaped. A section of shunt channel 6 is added on the sidewall of the channel and intersects with the original injection channel to form a bifurcation structure. The bifurcation structure can make the shunt channel 6 and the original injection channel symmetrically or asymmetrically distributed at a certain angle. The size is selected between 30° and 150° depe...

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Abstract

The present invention discloses a special microfluidic chip and a single-sampling continuous-separating electrophoresis method. The invention is applied in the technique field of the microfluidic chip in life science, medicine and analytical chemistry. The invention is characterized in that the invention comprises a method for exerting the electrophoresis high tension electricity parameter and a microfluidic chip which is used together with the method. The shunting driving voltage which is exerted to the microfluidic chip through an electrophoresis special high voltage power source leads to that the redundant buffer solution and sample solution totally flow into the shunting solution storage tank through a shunting channel. The side wall of the sampling channel on the special microfluidicchip is added with a shunting channel which forms a crossing structure with the former sampling channel and a shunting solution storage tank communicating with the shunting channel. The beneficial effects of the invention is effective prevention to the leakage of the buffer solution, avoidance to the dilution of the sample solution, evident reinforcement to the repeatability of a plurality of peak heights of the sample in the continuous-separation electrophoresis pattern and increased accuracy of the analyzing result of the continuous-electrophoresis separation experiment.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips used in life sciences, medicine, and analytical chemistry, and relates to a dedicated microfluidic chip and a single-time sample-loading continuous separation electrophoresis method, in particular to the ability to improve continuous separation electrophoresis An electrophoresis method for peak height stability and a microfluidic chip used in conjunction with the method. Background technique [0002] Microfluidic Chip (Microfluidic Chip), also known as Micro Total Analysis System (μ-TAS), integrates the processes of sample preparation, biochemical reaction, separation and detection involved in the fields of chemistry and biology into a digital square Centimeter-sized technology on a chip. When the electrophoresis is separated, a voltage is applied to both ends of the microchannel to control the flow direction and velocity of the fluid in the microchannel under the composite action of e...

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Application Information

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IPC IPC(8): G01N27/453B01D57/02B01L3/00B81B1/00
Inventor 刘冲陈刚王晓东徐征杜立群刘军山刘文涛
Owner DALIAN UNIV OF TECH
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