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Highly effective particle filter biological detection method and safe cabinet detection system

A detection method and filter technology, applied in biochemical equipment and methods, biological material sampling methods, methods for supporting/immobilizing microorganisms, etc., can solve structural leakage of high-efficiency particle filters, complex on-site operation instrument requirements, Problems such as structural leakage of materials, to achieve true and accurate detection results, safe and reliable detection process, and high specificity

Inactive Publication Date: 2008-11-05
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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Problems solved by technology

Therefore, the detection and evaluation results of the high-efficiency particle filter in the exhaust system of the negative pressure infection animal breeding isolation device by the existing method are often inconsistent with the actual filtering effect of the high-efficiency particle filter on microbial aerosols.
At the same time, the existing detection methods only perform progressive scan detection on the high-efficiency particle filter, and cannot systematically detect the structural leakage, damage leakage, and material structural leakage of the entire high-efficiency particle filter.
In addition, the physical detection method also has the characteristics of complex on-site operation and high requirements for instruments.

Method used

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  • Highly effective particle filter biological detection method and safe cabinet detection system

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Embodiment Construction

[0015] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0016] like figure 1 As shown, the secondary and tertiary biosafety cabinet detection systems in the present invention include a cabinet 1, and a high-efficiency particle filter 3 connected to the exhaust pipe 2 is set at the top air outlet of the cabinet 1, and the high-efficiency particle A microbial aerosol generator 4 and an air microbial sampler 5 are arranged at the front end of the filter 3, and 1 to 4 air microbial samplers 6 are arranged outside the cabinet 1, and the air inlet ends of each sampler 6 are respectively passed through a sampling The pipe 7 is connected to the exhaust pipe above the rear end of the high-efficiency particle filter 3, and the outlets of the samplers 4 and 5 are respectively connected to a suction pump through a pipeline. The front of the cabinet body 1 is provided with an open front window operation port 8, and th...

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Abstract

The invention relates to a high efficient particle filter biological detecting method and a safety cabinet detecting system. The front end of the high efficient particle filter at an exhaust outlet of the biological safety cabinet is provided with a microorganism aerosol generator and an air microorganism biological sampler; meanwhile, the rear end of the high efficient particle filter is provided with one to four air microorganism biological samplers, a microorganism culture dish is arranged inside each sampler; when the air is delivered to a front window operating opening of the cabinet body, the analog indicating microorganism is produced artificially, the analog indicating microorganism is the bacterium and virus which do not exist in the natural air and are not harmful to human, animals and the environment; the analog indicating microorganism is collected by the culture dishes arranged inside the front air microorganism biological sampler and the rear air microorganism biological sampler of the high efficient particle filter; the counting is realized after the culture; the filter efficiency of the efficient particle on the microorganism is obtained by counting the ratio of the pre-counting result to the post-counting result of the high efficient particle filter.

Description

technical field [0001] The invention relates to a biological detection method and a detection device, in particular to a biological detection method of a high-efficiency particle filter of a secondary and tertiary biological safety cabinet and a detection system of the safety cabinet. Background technique [0002] In pathogenic microbiology and biomedical experiments, secondary and tertiary biosafety cabinets are commonly used and must-have biosafety protection equipment in pathogenic microbiology laboratories, and are also primary protection for tertiary and quaternary biosafety laboratories The barrier, its exhaust air filtration system's filtration and purification effect on pathogenic microbial aerosols is directly related to the protection of the environment outside the laboratory. [0003] At present, the detection of high-efficiency particle filters (HEPA) in the exhaust system of secondary and tertiary biological safety cabinets at home and abroad is based on non-bio...

Claims

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Application Information

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IPC IPC(8): C12Q1/02G01N33/00C12M1/34C12M1/12C12M1/26B01L1/00
CPCC12M33/14C12M23/10
Inventor 李劲松温占波鹿建春赵建军毕建军
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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