Method for quantitatively testing halcinonide in cosmetics

A quantitative detection method, the technology of chlorofluxazone, applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of serious impurity interference and low detection sensitivity in cosmetics, so as to avoid interference and ensure correctness Effect

Inactive Publication Date: 2012-01-11
CHINA JILIANG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] 1. Low detection sensitivity: the detection limit of the UV spectrophotometer is generally at the milligram level;
[0007] 2. The ultraviolet spectrophotometer has no separation function, and the interference of impurities in cosmetics is serious

Method used

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  • Method for quantitatively testing halcinonide in cosmetics
  • Method for quantitatively testing halcinonide in cosmetics
  • Method for quantitatively testing halcinonide in cosmetics

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Embodiment 1

[0034] Embodiment 1, a quantitative detection method of clorfluxazone in a cosmetic, the cosmetic to be tested is a liquid cosmetic, and the following steps are carried out successively:

[0035] 1) Add 100% pure clofluazidone and Atractylodes lactone II to the chromatographic methanol, mix evenly to form a reference substance, the concentrations of clofluxazone and Atractylodes lactone II in the reference substance are 5 μg / mL respectively and 10 μg / mL.

[0036] 2), first add 0.2 mL of Atractylodes lactone II methanol solution as an internal standard in a dry plugged centrifuge tube, the concentration of Atractylodes lactone II methanol solution is 10 μg / mL, that is, each mL is composed of chromatographic methanol and Atractylodes lactone II The formed solution contained 10 μg of atractyloid II; then the centrifuge tube was blown dry with nitrogen flow in a water bath at 45°C; then 0.2 mL of the cosmetic to be tested was added to the centrifuge tube.

[0037] 3), according t...

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Abstract

The invention discloses a method for the quantitative detection of halcinonide in cosmetics, which comprises the steps that: 1) halcinonide and butenolide II are added into chromatographic methanol to obtain a reference substance; 2) a butenolide II methanol solution is added into a centrifuge tube and then cosmetics to be detected are added into the centrifuge tube which is dried in a manner of blow; 3) an extraction reagent is added; 4) centrifugation is carried out to obtain all organic phase solutes; 5) the organic phase solutes are dissolved in a mobile phase and a liquid layer is obtained after centrifugation; 6) the reference substance obtained by the step 1) and the liquid layer by the step 5) are carried out high efficiency liquid chromatographic analysis respectively; 7) the content of halcinonide in cosmetics to be detected is equal to the absorption peak area ratio of the halcinonide and the butenolide II in the liquid layer which is multiplied by the concentration of the halcinonide in the reference substance and divided by the absorption peak area ratio of the halcinonide and the butenolide II in the reference substance. The method for halcinonide detection provided by the invention has the advantage of high accuracy.

Description

technical field [0001] The invention relates to a method for the quantitative detection of glucocorticoid-cloflunixone in cosmetics. Background technique [0002] Halcinonide (Halcinonide) is a kind of glucocorticoid drug. Clinical studies have found that Halcinonide combined with calcipotriol for external use can effectively treat localized chronic eczema, with a total effective rate of 88.9%. Combined application with calcipotriol has a significant effect on the treatment of psoriasis vulgaris, and the average change rate of the psoriasis area severity index (PASI) is significantly higher than that of the two alone groups. At the same time, clonazepam used alone in the treatment of chemotherapy leakage injury has a good curative effect. In conclusion, clofluxazone has a good curative effect in the treatment of psoriasis and clinical common skin diseases. [0003] Cosmetics are closely related to people's daily life. In recent years, with the improvement of people's livin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74G01N30/02
Inventor 卢秀莲葛建楼纪东黄丽红孟庆莉徐春红周益峰罗小牛
Owner CHINA JILIANG UNIV
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