Active fruit and vegetable fresh-keeping film
A fruit and vegetable preservation and fresh-keeping film technology is applied in the directions of fruit and vegetable preservation, protection of fruits/vegetables with a protective layer, food preservation, etc. , No chemical pollution, good film-forming effect
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example 1
[0009] 1) Inoculate the Cryptococcus laurentii preserved on the slope into a 250mL Erlenmeyer flask containing 50mL of sterilized NYDB medium after activation on the slope, culture at a constant temperature of 20°C at 150r / min for 40h, and centrifuge the fermentation broth at 2500r / min for 10min Collect the cells, wash the yeast cells twice with sterile saline, count with a hemocytometer and adjust the concentration to 10 10 CFU / mL of yeast cell suspension.
[0010] 2) Take 1.0 g of sodium alginate, add it to 100 ml of distilled water, and stir evenly. Add 1.0g of glycerin, 0.1g of palmitic acid, 2g of monoglycerides, and 2g of β-cyclodextrin into the sodium alginate solution, and stir at 300r / min for 30min to form a uniform and stable emulsion.
[0011] 3) Add 5mL of yeast cell suspension into the sodium alginate emulsion, and stir evenly.
[0012] 4) Spray the sodium alginate composite film on the surface of the apples, dry them naturally or with cold wind, and store them ...
example 2
[0014] 1) Inoculate the yeast preserved on the slant into a 250mL Erlenmeyer flask containing 70mL of sterilized NYDB medium, cultivate it at a constant temperature of 25°C at 180r / min for 30h, and centrifuge the fermentation broth at 3500r / min for 8min to collect the bacteria. Wash the yeast cells twice with saline, count with a hemocytometer and adjust the concentration to 10 11 CFU / mL of yeast cell suspension.
[0015] 2) Take 1.0 g of sodium alginate, add it to 100 ml of distilled water, and stir evenly. Add 2.0g of glycerin, 0.5g of palmitic acid, 0.5g of monoglyceride, and 0.5g of β-cyclodextrin into the sodium alginate solution, and stir at 350r / min for 30min to form a uniform and stable emulsion.
[0016] 3) Add 2 mL of yeast cell suspension into the sodium alginate emulsion, and stir evenly.
[0017] 4) Spray the sodium alginate composite film on the surface of the strawberry, air dry it naturally or dry it with cold wind, and store it at room temperature.
example 3
[0019] 1) Inoculate the yeast preserved on the slant into a 250mL Erlenmeyer flask containing 100mL of sterilized NYDB medium, culture at 200r / min at a constant temperature of 30°C for 20h, and centrifuge the fermentation broth at 4000r / min for 5min to collect the bacteria, and use sterile physiological Wash the yeast cells twice with saline, count with a hemocytometer and adjust the concentration to 10 12 CFU / mL of yeast cell suspension.
[0020] 2) Take 2.0 g of sodium alginate, add it to 100 ml of distilled water, and stir evenly. Add 3.0g of glycerin, 1.0g of palmitic acid, 1.0g of monoglyceride, and 1.0g of β-cyclodextrin into the sodium alginate solution, and stir at 400r / min for 30min to form a uniform and stable emulsion.
[0021] 3) Add 1 mL of yeast cell suspension to the sodium alginate emulsion, and stir evenly.
[0022] 4) Spray the sodium alginate composite film on the surface of the cherry tomatoes, let it dry naturally or with cold wind, and then store it at ...
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