Preparation method for radionuclide <99>Tc<m> marked lactobacillus acidophilus

Abstract

The invention relates to a preparation method of lactobacillus acidophilus marked with a radioactive nuclide <99> technetium <m> of the lactobacillus acidophilus marked by adopting the radioactive species <99> technetium <m> (<99>Tc<m>). An MRS culture medium is used for resuscitating a vacuum cooling drying lactobacillus acidophilus seed under the anaerobic condition at 37 DEG C inside a super-clean bench; after passage cultures twice, then an LbS selective culture medium is used for culturing under the anaerobic condition at 37 DEG C; the lactobacillus acidophilus is gathered; brine is poured in a PYP bottle and shaken up; the liquid in the bottle is taken out, then is poured into a second bottle, and can be continuously poured in a plurality of bottles; the final liquid is taken out, poured in the bottle for containing germ, and shaken up; the bottle for containing the germ is put in a incubator for incubation; the bottle is taken out, washed with the brine, and centrifuged; the bottle is washed again and centrifuged for more times; supernatant fluid is thrown away, <99>Tc<m>O4 liquid or liquid with bigger radioactivity is added in a lactobacillus acidophilus bottle and is put in the incubator for incubation; the washing and the centrifuging for more times are performed repeatedly; and the supernatant fluid is thrown away, and precipitate is measured with a radioactivity meter.

Description

technical field [0001] The present invention uses radionuclide 99 Technetium m ( 99 Tc m ) a radionuclide that labels Lactobacillus Acidophilus 99 Technetium m Preparation method of labeled Lactobacillus acidophilus. It relates to the field of medical preparations and preparation of pharmaceuticals. Background technique [0002] In the field of medicine, radiopharmaceutical tracer technology is widely used to diagnose, treat and study diseases. Currently used radionuclides 99 Technetium m ( 99 Tc m ) labeled drugs can be divided into three categories from the perspective of preparation: [0003] 1. 99 Technetium m ( 99 Tc m ) complex 2. 99 Technetium m ( 99 Tc m ) marker protein 3. 99 Technetium m ( 99 Tc m ) labeled colloid [Experimental Nuclear Medicine and Nuclear Pharmacy (Chapter Five Radiopharmaceuticals, Section Two Radiotechnetium Labeled Drugs) p. 100]. Radiopharmaceuticals are basically administered intravenously, and some are directly orall...

AI Technical Summary

Problems solved by technology

Radiopharmaceuticals are basically administered intravenously, and there are also direct oral methods, but the direct oral method has the disadvantage of being partially absorbed by the gastric mucosa, and all bacterial inspections and studies are carried out in vitro

As a result, studies and testing have been less effective