Coloring agent for measuring urine tangible component by flow type cell principle

Abstract

The utility model relates to a coloring agent used for urine analysis, in particular to a coloring agent used for mensurating urine formed components by a flow cell principle, and comprises the following components with the weight percentage as follows: 95.81 to 97.88 percent of ethylene glycol, 2 to 4 percent of methanol, 0.11 to 0.15 percent of dye I, and 0.01 to 0.04 percent of dye II. The coloring agent used for mensurating the urine formed components by the flow cell principle belongs to fluorescent dye and has the advantages of high accuracy of analysis results and good stability; the coloring agent is particularly applicable to the flow cell principle.

Description

technical field [0001] The invention relates to a staining agent for urine analysis, in particular to a staining agent for measuring urine formed components by flow cytometry. Background technique [0002] In the detection and analysis of the high-end urine sediment analyzer, the principles of flow cytometry and electrical impedance analysis are applied: after the cells in the urine are dyed with fluorescent dyes, under the action of the sheath fluid, a single, columnar cell flow is formed, and the cells pass through quickly. In the argon laser detection area, the instrument detects changes in fluorescence, scattered light and electrical impedance. The forward scattered light signal intensity and fluorescence intensity are used to distinguish small cells such as red blood cells, white blood cells, and bacteria; the forward scattered light pulse width is used to distinguish large cells such as epithelial cells and casts. The application of fluorescent dyeing technology in hi...

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Problems solved by technology

[0003] Due to the current problems of the dye solution used in the principle of flow cytometry, the reagent formulation is difficult, the cost is high, the long-term dependence on imports, and the purchase channels are cumbersome, etc., which cannot meet the normal use of the hospital in time.

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