Fluorescent quantitative PCR detection system based on bottom scan detection

Abstract

The invention discloses a fluorescence quantitative PCR detecting system based on bottom scanning detection, comprising a temperature changing metal module capable of being inserted with a plurality of test tubes, a semiconductor freezer and a radiating device which are arranged into a whole in sequence from up to bottom. The system is characterized in that test tube slots of the temperature changing metal module are through holes running through the temperature changing metal module, the semiconductor freezer and the radiating device; the radiating device is a thermotube radiator, the thermotube extends to the outside of the whole structure, the extending end is connected with a radiator fin; a photoelectric detector is positioned at the bottom of the radiating device of the whole structure and is driven to scan by an electric motor; and the reagent fluorescence is detected from the bottom of the test tubes. The adoption of the system contributes to improving the luminous efficiency, obtaining higher fluorescence sensitivity and the fluorescence signal intensity, and improving the stability, ensures that the detection result is more accurate, realizes the rapid temperature rise and temperature reduction as well as the accurate temperature control of the temperature changing metal module, and greatly prolongs the service life of the temperature changing metal module.

Description

technical field [0001] The invention relates to a fluorescent quantitative PCR detection system, in particular to a fluorescent quantitative PCR detection system based on bottom scanning detection. Background technique [0002] The working principle of the fluorescent quantitative PCR detection system is an instrument that automatically cycles the reactant between the specified denaturation temperature, annealing temperature and extension temperature. Through the temperature cycle of denaturation, annealing and extension, the target DNA can be amplified in a short time Millions of times. [0003] The current mainstream fluorescent quantitative PCR detection system includes a temperature-variable metal module that can be inserted into several test tubes, a semiconductor refrigerator, a heat dissipation device and a fan, which are installed sequentially from top to bottom. The semiconductor refrigerator is used to change the temperature, so that the temperature of the tempera...

AI Technical Summary

Problems solved by technology

But the disadvantage is: due to the uneven top of the plastic test tube, evaporation and condensation water vapor often stays and drips, resulting in sudden changes in the fluorescence signal and poor detection repeatability

In this mode, the influence of the plastic test tube can be reduced, but the optical fiber located on the side wall of the module is easily polluted by residues, worn, broken, and difficult to clean, and its use is limited and its lifespan is shortened

I believe that the signal in this mode is relatively weak and the system stability is poor

And it is very difficult to bury and maintain the optical fiber

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