Rooting method of tissue culture and rapid propagation of eucalyptus dunni

A technology of tissue culture rapid propagation and rooting medium, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of difficult rooting of tissue culture seedlings, and meet the needs of large-scale production, increase the proliferation coefficient, and proliferate The effect of increasing the coefficient

Inactive Publication Date: 2008-12-31
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the purpose of the present invention is to provide a rooting method for

Method used

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  • Rooting method of tissue culture and rapid propagation of eucalyptus dunni
  • Rooting method of tissue culture and rapid propagation of eucalyptus dunni
  • Rooting method of tissue culture and rapid propagation of eucalyptus dunni

Examples

Experimental program
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Example Embodiment

[0033] Example 1

[0034] Select Eucalyptus Dunn seeds that are full and disease-free, put them in a 1.5ml centrifuge tube, rinse with tap water and soak for 10 minutes, sterilize with 70% ethanol for 30 seconds, then sterilize with 0.1% mercuric chloride for 2 minutes, and then wash with sterile water 5 times, inoculate MS+0.5mg / L 6-BA+5g / L agar+30g / L sucrose germination medium (pH 5.8) under aseptic conditions. The culture conditions are 25℃ in day and 18 in night. ℃, light intensity 40μmol·m -2 ·S -1 ,Illumination time 14h·d -1 (The same under the culture conditions), 8 days later, the seeds germinated and sterile seedlings were obtained.

[0035] Cut the sterile seedling from the base of the cotyledon, and transfer the cotyledon to MS+0.5mg / L 6-BA+0.5mg / L KT+0.25mg / LIBA+5g / L agar+30g / L sucrose (pH 5.8) or MS+0.5mg / L ZT+0.25mg / L IAA+5g / L agar+30g / L sucrose (pH 5.8) proliferation medium, under the above conditions for day and night light cycle culture, 9 days later induced forma...

Example Embodiment

[0038] Example 2

[0039] Select Eucalyptus Dunn seeds that are full and disease-free, put them in a 1.5ml centrifuge tube, rinse with tap water, soak for 10 minutes, sterilize with 70% ethanol for 20 seconds, then sterilize with 0.1% mercuric chloride for 3 minutes, and then wash with sterile water 6 times, inoculate MS+0.3mg / L 6-BA+5g / L agar+30g / L sucrose germination medium (pH 5.8) under aseptic conditions. The culture conditions are day temperature 25℃, night temperature 18 ℃, light intensity 40μmol·m -2 ·S -1 ,Illumination time 14h·d -1 (The same under the culture conditions), 6 days later, the seeds germinated and sterile seedlings were obtained.

[0040] Cut the sterile seedling from the base of the cotyledon, and transfer the cotyledon to MS+0.45mg / L 6-BA+0.6mg / L KT+0.3mg / LIBA+5g / L agar+30g / L sucrose (pH 5.8) or MS+0.45mg / L ZT+0.3mg / L IAA+5g / L agar+30g / L sucrose (pH 5.8) proliferation medium, under the above conditions for day and night light cycle culture, 11 days later i...

Example Embodiment

[0043] Example 3

[0044] Select Eucalyptus Dunn seeds that are full and free from diseases and insect pests, put them in a 1.5ml centrifuge tube, rinse with tap water, soak for 10 minutes, sterilize with 70% ethanol for 40 seconds, then sterilize with 0.1% mercuric chloride for 1 minute, and then wash with sterile water 6 times, inoculate MS+5g / L agar+30g / L sucrose germination medium (pH 5.8) under aseptic conditions. The culture conditions are day temperature 25℃, night temperature 18℃, light intensity 40μmol·m -2 ·S -1 ,Illumination time 14h·d -1 (The same under the culture conditions), 7 days later, the seeds germinated and sterile seedlings were obtained.

[0045] Cut the sterile seedling from the base of the cotyledon, and transfer the cotyledon to MS+0.4mg / L 6-BA+0.5mg / L KT+0.6mg / LIBA+6g / L agar+35g / L sucrose (pH 5.8) or MS+0.4mg / L ZT+0.2mg / L IAA+6g / L agar+35g / L sucrose (pH value 5.8) in the proliferation medium, under the above conditions for day and night light cycle cultu...

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Abstract

The invention discloses a Eucalyptus dunnii group culture fast propagation rootage method, which comprises the following steps: (1) asepsis seedling cultivation; (2) bud subculture multiplication; (3) test-tube seedling rejuvenation; (4) inducing rootage; (5) transplanting the test-tube seedling, etc. In the invention, after the multiplication cultivation and rejuvenation cultivation, the asepsis seedling is inoculated in a first rootage culture medium and a second rootage culture medium for inducing a complete plant, thus solving the problem that the propagation coefficient of Eucalyptus dunnii group culture is low, in particular to the problem that the rootage is difficult; by utilizing the technical proposal of the invention, a large number of seedlings can be provided in a short time so as to replace the expensive imported seeds and satisfy the heavy demand of the large-scaled industrial seedling and the market.

Description

technical field [0001] The invention relates to a method for rooting Eucalyptus dunen, in particular to a rooting method for rapid propagation of Eucalyptus dunne by using tissue culture, and belongs to the technical field of plant tissue culture. Background technique [0002] Eucalyptus dunnii Maiden is a Myrtaceae genus Eucalyptus, subgenussymphyomyrtus, blue eucalyptus group (section Maidenaria) multi-branched eucalyptus (series Viminales), native to Australia, fast growing, straight trunk , uniform wood thickness, straight texture, drought resistance, disease resistance, strong cold resistance and other characteristics, can be used as particle board, pulp wood, wood sheet and other purposes, and is an excellent provenance for promoting and expanding the planting range of eucalyptus plantations . However, judging from the current market situation, there are still many problems to be solved in the large-scale planting of Dunn's Eucalyptus in China. Due to the limited dis...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 夏阳燕丽萍王太明刘翠兰李双云
Owner SHANDONG FOREST SCI RES INST
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