Rooting method of tissue culture and rapid propagation of eucalyptus dunni
A technology of tissue culture rapid propagation and rooting medium, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of difficult rooting of tissue culture seedlings, and meet the needs of large-scale production, increase the proliferation coefficient, and proliferate The effect of increasing the coefficient
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Embodiment 1
[0034] Select plump Eucalyptus Dunn seeds that are free from diseases and insect pests, put them in a 1.5ml centrifuge tube, rinse with tap water, soak for 10 minutes, sterilize with 70% ethanol for 30 seconds, then sterilize with 0.1% mercuric chloride for 2 minutes, and then wash with sterile water 5 times, inoculated into MS+0.5mg / L 6-BA+5g / L agar+30g / L sucrose germination medium (pH 5.8) under aseptic conditions, the culture conditions were daytime temperature 25°C, night temperature 18°C ℃, light intensity 40μmol·m -2 ·s -1 , light time 14h·d -1 (The same under the culture conditions), the seeds germinated after 8 days, and the aseptic seedlings were obtained.
[0035] Cut the sterile seedlings from the base of the cotyledons, and transfer the cotyledons to MS+0.5mg / L 6-BA+0.5mg / L KT+0.25mg / LIBA+5g / L agar+30g / L sucrose (pH 5.8) or In MS+0.5mg / L ZT+0.25mg / L IAA+5g / L agar+30g / L sucrose (pH value 5.8) proliferation medium, carry out day and night light cycle culture under...
Embodiment 2
[0039] Select plump Eucalyptus Dunn seeds that are free from diseases and insect pests, put them in a 1.5ml centrifuge tube, rinse with tap water, soak for 10 minutes, sterilize with 70% ethanol for 20 seconds, then sterilize with 0.1% mercuric chloride for 3 minutes, and then wash with sterile water 6 times, inoculated into MS+0.3mg / L 6-BA+5g / L agar+30g / L sucrose germination medium (pH 5.8) under sterile conditions, the culture conditions were daytime temperature 25°C, night temperature 18°C ℃, light intensity 40μmol·m -2 ·s -1 , light time 14h·d -1 (The same under the culture conditions), after 6 days, the seeds germinate to obtain sterile seedlings.
[0040] Cut the sterile seedlings from the base of the cotyledon, and transfer the cotyledon to MS+0.45mg / L 6-BA+0.6mg / L KT+0.3mg / LIBA+5g / L agar+30g / L sucrose (pH 5.8) or In MS+0.45mg / L ZT+0.3mg / L IAA+5g / L agar+30g / L sucrose (pH value 5.8) proliferation medium, carry out day and night light cycle culture under the above cond...
Embodiment 3
[0044] Select plump Eucalyptus Dunn seeds that are free from diseases and insect pests, put them in a 1.5ml centrifuge tube, rinse with tap water, soak for 10 minutes, sterilize with 70% ethanol for 40 seconds, then sterilize with 0.1% mercuric chloride for 1 minute, and then wash with sterile water 6 times, inoculated into MS+5g / L agar+30g / L sucrose germination medium (pH 5.8) under sterile conditions, the culture conditions were daytime temperature 25°C, night temperature 18°C, light intensity 40μmol·m -2 ·s -1 , light time 14h·d -1 (The same under the culture conditions), the seeds germinated after 7 days, and the aseptic seedlings were obtained.
[0045] Cut the sterile seedlings from the base of the cotyledon, and transfer the cotyledon to MS+0.4mg / L 6-BA+0.5mg / L KT+0.6mg / LIBA+6g / L agar+35g / L sucrose (pH 5.8) or In MS+0.4mg / L ZT+0.2mg / L IAA+6g / L agar+35g / L sucrose (pH value 5.8) proliferation medium, carry out day and night light cycle culture under the above conditions...
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