The invention discloses a two-step
seedling quick propagation method for hemerocallis
tissue culture by using tender
pedicel as explant. The method comprises the following steps of: sterilizing the tender hemerocallis
pedicel serving as the explant, then
cutting the
pedicel into sections of 1 to 1.5 centimeters, and inoculating the
cut pedicel to an induction and differentiation culture medium; transferring sterile seedlings with the height of 4 to 6 centimeters and over 4 leaves to a rooting culture medium after 2.5 to 3 months to perform rooting culture medium; and when each
tissue culture seedling grows over 3 roots and the adventitious root reaches a length of 2 to 4 centimeters, opening a
bottle cap, hardening the seedlings for 3 to 4 days indoors, taking the
tissue culture seedlings out of a culture
bottle, washing the culture medium, and
transplanting the seedlings to the sterilized culture medium, wherein the induction and differentiation culture medium: MS is used as a basic culture medium; 2 to 10 mg of 6-benzyladenine (6-BA), 0 to 2 mg of 2,4-dichlorphenoxyacetic acid (2,4-D), 0.2 to 1 mg of alpha-
naphthylacetic acid (NAA), 4.5 g of
agar and 30 g of
cane sugar are added into each
liter of basic culture medium; and the pH value of the culture medium is adjusted to 5.8. The rooting culture medium: 1 / 2MS is used as a basic culture medium; 0.1 to 2.0 mg of NAA, 4.5 g of
agar and 15 to 30 g of
cane sugar are added into each
liter of basic culture medium; and the pH value of the culture medium is adjusted to 5.8. The method can massively propagate good-quality hemerocallis tissue culture seedlings in short time so as to meet the requirement of large-scale production, and reduces the steps of preparing the culture medium and transferring the
bottle, so the method is simple and feasible and reduces the production cost.