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Degradation strain capable of high-efficiency degrading bactericide chlorothalonil and use thereof

A technology of chlorothalonil and fungicides, applied in the biological field, can solve problems such as pollution and soil entry, achieve high-efficiency degradation capabilities, and avoid secondary pollution problems

Inactive Publication Date: 2009-01-07
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the main application object of chlorothalonil is the leaf disease of crops, when the crop canopy is thin and excessively applied, most of it enters the soil and causes serious pollution (Cisar and Snyder, 1996; Petrovicet al., 1996)

Method used

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  • Degradation strain capable of high-efficiency degrading bactericide chlorothalonil and use thereof
  • Degradation strain capable of high-efficiency degrading bactericide chlorothalonil and use thereof

Examples

Experimental program
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Effect test

specific Embodiment 1

[0019] Specific Example 1: Strain TP-D1 grows with chlorothalonil as carbon source and degrades chlorothalonil

[0020] 0.5mL of TP-D1 bacterial suspension (1×10 8 cells / mL) were inserted into the inorganic salt culture solution containing 50 mg / L chlorothalonil, and repeated three times, and the inorganic salt culture solution containing the same concentration of chlorothalonil without inoculating bacteria was used as the control. 30°C, 180 rpm shaking culture, culture for 24, 48, 72, 96 hours, respectively, gas chromatography to detect the content of chlorothalonil. The results showed that after inoculating TP-D1, the residue of chlorothalonil in the culture medium gradually decreased, and its residue was only 4.5 mg / L in 96 hours, and the degradation rate of chlorothalonil reached 90.4% (Table 1).

[0021] Table 1 Degradation rate of chlorothalonil by bacterial strain TP-D1 in inorganic salt culture solution

[0022]

specific Embodiment 2

[0023] Specific Example 2: Degradation of chlorothalonil in soil by bacterial strain TP-D1

[0024] Pass the soil through a 0.2mm sieve, dry heat sterilization at 160°C for 1.5 hours, weigh 10g and put it in a 90mm petri dish, spray chlorothalonil mother liquor, so that the initial concentration of chlorothalonil in the soil is 50mg / L, Evenly mix the TP-D1 bacterial solution cultivated for 36 hours into the soil to make the concentration 10 7 Each gram of soil, soil moisture 20%, set 3 times to repeat, take the soil that adds medicine but does not add bacteria as control. The treated soil was incubated at room temperature, and samples were taken to detect the residual amount of chlorothalonil in the soil 1, 3, and 7 days after the treatment. At the same time, attention should be paid to spraying water the next day to keep the humidity at about 20%. The results showed that after the bacterial strain was cultured in soil for 7 days, the degradation rate of chlorothalonil could ...

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Abstract

The invention relates to a degrading bacteria which can effectively degrade a disinfectant of chlorothalonil. The classified name of the degrading bacteria is: Ochrobactrum lupine which is preserved in China General Microbiological Culture Collection Center in June the 28th, 2007 and the preservation number thereof is CGMCC No.2098. The degrading bacteria is used to degrade the disinfectant of chlorothalonil and can be used for the biological purifying of water, soil or agricultural products polluted by the chlorothalonil.

Description

technical field [0001] The invention belongs to the field of biotechnology, more precisely, relates to a degrading bacterium capable of efficiently degrading the fungicide chlorothalonil and its application technical background [0002] As an agricultural fungicide, chlorothalonil is mainly used to prevent and control various fungal diseases. According to the statistics of the USGS (United States Geological Survey), the annual usage of chlorothalonil in the United States in recent years has exceeded 4.8×10 6 Kilogram. The annual production capacity of chlorothalonil in my country has exceeded 8,000 tons. Although the main application object of chlorothalonil is the leaf disease of crops, when the crop canopy is thin and excessively applied, most of it enters the soil and causes serious pollution (Cisar and Snyder, 1996; Petrovicet al., 1996) . There is evidence that chlorothalonil can be transported over long distances and its presence has been monitored in the Arctic Cir...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C09C1/10A61K48/00A61K38/43
Inventor 郭荣君李世东史秀珍
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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