Method for on-line detection for solid-state biomass bioconversion procedure
A technology for biotransformation and biomass, applied in the field of instrument analysis, to achieve good economic benefits, applicability, and ease of use
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[0016] Example 1 Using Near Infrared Spectroscopy to Quickly Determine the Water Content of the Medium in the Process of Solid Fermentation
[0017] The process of the embodiment of the present invention is as follows:
[0018] The first step is the preparation of the modeling sample set. Steam popcorn and wheat bran are used as the main medium for solid-state fermentation, and the medium is adjusted to between 45-85%. After the medium is sterilized, it is inoculated with Trichoderma viride at 30℃ Solid-state fermentation is carried out in a gas-phase dual-dynamic solid-state fermentation reactor. After a certain period of incubation, it is used as a calibration set sample for the near-infrared multiple regression prediction model, and the fermentation sample is taken out. The number of samples is better than 50. The representativeness of the sample set has a great influence on the stability and adaptability of the prediction model. It is better that the standard deviation of the ...
Example Embodiment
[0024] Example 2 Using Near Infrared Spectroscopy to Quickly Determine the Biomass of the Medium in the Solid State Fermentation
[0025] The process of the embodiment of the present invention is as follows:
[0026] The first step is the preparation of the modeling sample set. Steam-exploded corn stover and wheat bran are used as the main medium for solid-state fermentation, and the water content of the medium is adjusted to about 75%. After the medium is sterilized, it is inoculated with Penicillium decumbens at 30℃ Carry out solid-state fermentation, after culturing for a certain period of time, take out the fermentation sample and use it as the calibration set sample for the near-infrared multiple regression prediction model. The number of samples is preferably 50 copies.
[0027] The second step is to measure the near-infrared spectra of solid fermentation samples. In this embodiment, a Nexus Fourier Transform Near-Infrared Spectrometer produced by Nicolet Company of the Unit...
Example Embodiment
[0032] Example 3 Using Near Infrared Spectroscopy to Quickly Determine the Cellulase in the Culture Medium During the Solid State Fermentation
[0033] The process of the embodiment of the present invention is as follows:
[0034] The first step is the preparation of the modeling sample set. Steam-exploded corn stalks are used as the main medium for solid-state fermentation. The water content of the medium is adjusted to about 75%. After the medium is sterilized, it is inoculated with Trichoderma viride for solid-state fermentation at 30°C. After incubating for a certain period of time, the fermentation samples were taken out and used as the calibration set samples for the near-infrared multiple regression prediction model. The number of samples is preferably 50 copies.
[0035] The second step is to measure the near-infrared spectra of solid fermentation samples. In this embodiment, a Nexus Fourier Transform Near-Infrared Spectrometer produced by Nicolet Company of the United Sta...
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