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Tissue-patching material for injection and preparation method thereof

A repair material and injection technology, which is applied in the field of injectable tissue repair materials and its preparation, can solve the problems of unseen product applications and literature reports, etc., to promote the growth of fibroblasts, accelerate tissue repair, and promote damage healing effect

Inactive Publication Date: 2009-01-21
西安组织工程工程技术研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] After searching, there is no report on the application and literature of this kind of product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] Example 1: Injectable skin tissue repair material

[0017] Step 1). Expansion and culture of fibroblasts: Aseptically obtain allogeneic skin tissue, remove the fat layer, cut into a size of 5mm×10mm, digest with neutral protease, separate the epidermis and dermis tissue, and digest the dermis with 600U / ml collagenase Tissue for 1 hour; after digestion, the dermal tissue was blown away to collect the cell fluid, and after centrifugation, the supernatant was discarded to obtain fibroblasts; after adding fibroblast culture medium, the cells were suspended by blowing, inoculated into culture bottles to supplement the culture medium, and cultured to the required number , collect the cell suspension as fraction A.

[0018] Step 2). The preparation method of basic fibroblast growth factor (bFGF) slow-release microspheres can refer to the literature ("Preparation of composite bFGF slow-release microspheres and its experimental research on fibroblast proliferation" Biomedical En...

example 2

[0021] Example 2: Preparation of Injectable Adipose Tissue Repair Material

[0022] Step 1). Expansion culture of adipocytes: Aseptically obtain autologous adipose tissue, cut into 1mm 3 Size, after digestion with 300U / ml collagenase solution for 1 hour, the adipose tissue was blown away, the cell solution was collected, and the supernatant was discarded after centrifugation to obtain adipocytes; after adding adipocyte culture medium, the cells were blown and suspended, and inoculated into a culture bottle to supplement the culture cultured to the required amount, and the cell suspension was collected as component A.

[0023] Step 2). Flurbiprofen axetil lipid microspheres (produced by Beijing Tide Pharmaceutical Co., Ltd., have anti-inflammatory, analgesic and antipyretic effects, and are clinically used for the treatment of pain or inflammatory diseases) are used for standby.

[0024] Step 3). Preparation of injectable adipose tissue repair material: dissolve fibrin, collag...

example 3

[0026] Example 3: Preparation of Injectable Cartilage Tissue Repair Material

[0027] Step 1). The expansion culture of chondrocytes: Aseptically obtain allogeneic cartilage tissue, cut into small grain size, digest the cartilage tissue with 400U / ml collagenase solution, blow the cartilage tissue to collect the cell fluid, centrifuge and discard the upper After adding chondrocyte culture medium, the cells were blown to suspend, inoculated into a culture bottle to supplement the culture medium, cultured to the required number, and the cell suspension was collected as component A.

[0028] Step 2). Preparation of insulin-like growth factor 1 (IGF-1) slow-release microspheres: the preparation of slow-release microspheres is carried out according to Example 1; 0.1 g of dry microspheres is swollen in 10 ml double-distilled water containing 10 μg IGF-1 After soaking for one day and freeze-drying, 60 Co irradiation sterilization spare.

[0029] Step 3). Preparation of injectable ca...

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Abstract

The invention relates to an injectable tissue patching material, and the material is characterized in that after injected to a recipient area, the mixture of viable cells, drug control releasing microspheres and gel can be shaped rapidly, filling up the impairment of soft tissues and promoting damage healing. Compared with the prior art, the injectable tissue patching material prepared in the invention has the advantages of high biocompatibility, rapid and free shaping and filling up the impairment of organs and tissues rapidly. The material can promote growth of fibrous cells, formation of neoformative blood capillaries, generation of granulation tissues and damage healing. The contained growth factors that are released continuously for a long time or drugs that promote healing can promote in vivo cells or implanted cells to produce bigger restoration function.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering medical materials, in particular to an injectable tissue repair material and a preparation method thereof. Background technique [0002] At present, in the repair of tissue defects, non-surgical treatment has grown rapidly, and the proportion has exceeded 80%. Among them, soft tissue filler injection is an important means of non-surgical treatment. With the continuous development of science and technology, there are more and more soft tissue filling materials, such as silica gel, collagen, polyacrylamide, hydrogel, etc. However, these filling materials have the disadvantages of low biocompatibility, easy degradation after implantation, and difficulty in maintaining long-term filling effects. [0003] Cells in the body can synthesize and secrete extracellular matrix, which can promote the synthesis and cross-linking of collagen molecules, and restore damaged tissues. Therefore, the use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/38A61L27/52A61L27/54A61L27/50
Inventor 黄馨慧
Owner 西安组织工程工程技术研究中心
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