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Preparation of camellia flavonol

A technology of camellia flavonoids and flavonoids is applied in the field of extraction and purification of flavonoids to achieve the effects of good color and luster, good quality and low cost

Inactive Publication Date: 2011-09-21
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the medicinal value has not been reported at home and abroad

Method used

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  • Preparation of camellia flavonol
  • Preparation of camellia flavonol
  • Preparation of camellia flavonol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～23

[0030] According to the specific operation steps above, the camellia flavonoids are extracted under the set conditions, the extraction solvent is ethanol-water solution of different concentrations, and the yield calculation method of flavonoids is:

[0031] Flavonoid yield = (mass of flavonoids in the extract / mass of Nanshan tea seed meal) × 100%

[0032] The specific condition setting and extraction results are shown in Table 1:

[0033] Table 1

[0034]

Embodiment 24

[0036] This embodiment is an orthogonal experiment to investigate the extraction process. The extraction process steps are the same as those in the aforementioned Examples 1 to 23. According to the results of the aforementioned Examples 1 to 23, 3 appropriate levels are selected for the 4 factors respectively, and L 9 (3 4 ) Orthogonal experiment schedule to investigate the optimal process conditions. The horizontal arrangement of the factors in the orthogonal experiment is shown in Table 2, and the experimental results are shown in Table 3:

[0037] Table 2

[0038]

[0039] table 3

[0040]

[0041] The analysis shows that the influence of each factor is in the order of D>B>A>C, that is, extraction solvent times>extraction time>extraction solvent concentration>extraction temperature, so the best process combination is A 2 B 3 C 1 D. 2 , that is, 20 times the amount of 70% ethanol-water as a solvent, 80 ℃, extraction 2 times, 2.5 hours each time, the flavonoid con...

Embodiment 25～29

[0048] Get 2 grams of the extract extract prepared by the method of any one of Examples 1 to 24, dissolve in an appropriate amount of water, filter, and put DiaionHP-20 macroporous adsorption resin column (resin dry weight 20g) on ​​the filtrate, after the adsorption is completed Rinse with deionized water, then carry out gradient elution with 10%, 20%, 30%, 40%, and 50% ethanol-water solution at 2BV / h, enrich the eluent, and concentrate it into a paste with a rotary evaporator. Then place it in a vacuum oven at 60°C and dry to constant weight, measure the flavonoid content and calculate its elution rate:

[0049] Wherein, the calculation method of the elution amount of the extract is the weight of the eluent.

[0050] The calculation method of the elution rate of flavonoids is: (amount of flavonoids in eluent) / (amount of flavonoids in loading extract)×100%.

[0051] The specific conditions and results are shown in Table 4.

[0052] Table 4

[0053]

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Abstract

A method for preparing camellia flavone is provided. The method comprises the following steps that firstly pressed Nanshan tea seeds meal is used as the raw material to carry out solvent extraction for 1 to 5 hours at a temperature of 6 to 100 DEG C, with an extraction solvent being an ethanol-water solution having a concentration of 60 to 90 percent, a material to liquid ratio (g, ml) of 1:5-25,and 1 to 4 extractions are carried out; secondly the extract is condensed, dried, dissolved in water, and then filtered, the filtrate is adsorbed by DiaionHP-20 large-hole resin, a deionized water is used to elute impurities, an ethanol-water solution with a concentration of 20 to 50 percent is used for eluting, and then the elute is condensed and dried to obtain the product of camellia flavone. The camellia flavone prepared with the method provided by the invention has high purity, good quality, and good color; the content of flavone after purification of the extract exceeds 50 percent and satisfies the content standard for development and registration of class-5 new drugs; the method is easy to carry out with low cost and the non-toxic solvent; the large-hole resin is renewable, recyclable, and suitable for large-scale production.

Description

technical field [0001] The invention relates to a method for extracting and preparing active ingredients in natural plant camellia japonica seeds, in particular to a method for extracting and purifying flavonoids. Background technique [0002] Camellia semiserrata Chi (Camellia semiserrata Chi) is a plant of the genus Camellia in the family Theaceae. It is distributed in the southeastern part of Guangxi to the southwestern part of Guangxi, at an altitude of 250 to 500 meters. It is an important oil tree species rich in resources. The quality is good, the leaves are beautiful, and the flowers are huge. As oil and flower species, it is also cultivated in large quantities in addition to wild. But the medicinal value has not been reported at home and abroad so far. [0003] The inventor's research on Camellia began in 2002, and successively went to Guangxi, Yunnan, and Hunan, the main producing areas of Camellia, to conduct resource investigations. On the basis of the resource...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08A61K36/82
Inventor 王永奇唐玲王威冯宝民
Owner DALIAN UNIV
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