Method for detecting chlorpromazine and special ELISA kit thereof

An enzyme-linked immunosorbent assay, chlorpromazine technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unsuitable screening of samples for on-site monitoring, high technical requirements of inspectors, and cumbersome detection process, etc. To achieve the effect of shortening the detection time, easy to use, and low pre-processing requirements
CN101358967AInactive Publication Date: 2009-02-04BEIJING WANGER BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BEIJING WANGER BIOTECH
Publication Date
2009-02-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a method of detecting chlorpromazine, and a special enzyme linked immunosorbent kit thereof. The enzyme linked immunosorbent kit comprises haptens and specific antibodies of the chlorpromazine; and the specific antibodies are polyclonal antibodies or monoclonal antibodies of the chlorpromazine. The kit adopts the high specificity monoclonal antibodies of the chlorpromazine, which guarantees the reliability of detection results, and the experimental results show that the kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like; and the main reagents of the kit all adopt the form of working liquid, the operation is convenient, and the cost is low. The operation of the method which adopts the kit to detect the chlorpromazine is simple, the operation has low requirements for the pretreatment of the sample, and the method can be used for simultaneously and rapidly detecting samples in bulk. Therefore, the method which adopts the enzyme linked immunosorbent kit for detection can perform on-site monitoring and is suitable for qualitative and quantitative screening of the samples in bulk.
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Description

technical field

[0001] The invention relates to a method for detecting chlorpromazine and a special ELISA kit. Background technique

[0002] Chlorpromazine is a phenothiazine drug with the structural formula figure 1 As shown, it is a blocker of central dopamine polymers. This kind of medicine is added to the feed, and the livestock and poultry will sleep after eating, so as to reduce feed consumption and gain weight quickly. Human consumption of livestock and poultry products containing such drugs will also have the same effect, causing lethargy, obesity, and slow thinking. Countries such as Europe and the United States have banned or strictly banned the use of chlorpromazine. According to the No. 235 document of the Ministry of Agriculture of my country, the residue limit of chlorpromazine in food of animal origin is not allowed to be detected. Therefore, in practice, a method for detecting chlorpromazine with high accuracy and high sensitivity is needed.

[0003] At p...

Claims

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