Method for detecting chlorpromazine and special ELISA kit thereof

An enzyme-linked immunosorbent assay, chlorpromazine technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unsuitable screening of samples for on-site monitoring, high technical requirements of inspectors, and cumbersome detection process, etc. To achieve the effect of shortening the detection time, easy to use, and low pre-processing requirements

Inactive Publication Date: 2009-02-04
BEIJING WANGER BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the conventional detection methods of chlorpromazine residues mainly include liquid chromatography/mass spectrometry (LC/MS), high performance liquid chromatography (HPLC), etc., but these metho

Method used

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  • Method for detecting chlorpromazine and special ELISA kit thereof
  • Method for detecting chlorpromazine and special ELISA kit thereof
  • Method for detecting chlorpromazine and special ELISA kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1. Preparation and use of a kit using a hapten-carrier protein conjugate as a coating source and an enzyme-labeled secondary antibody as an enzyme marker

[0037] 1. The detection principle of the kit with the hapten and carrier protein conjugate as the coating source and the enzyme-labeled secondary antibody as the enzyme label is as follows:

[0038] When the coating on the microwell strip of the microtiter plate is originally a hapten-carrier protein conjugate, after adding the standard solution or sample solution to the microwell of the microtiter plate, add the specific antibody of chlorpromazine, and the sample The residual chlorpromazine competes with the chlorpromazine-coupled antigen on the ELISA plate for the chlorpromazine-specific antibody, and then the enzyme-labeled anti-antibody is added for amplification, and the color is developed with a chromogenic solution. The absorbance value of the sample is related to the content of chlorpromazine Negative...

Embodiment 2

[0138] Embodiment 2, the test kit that is used to detect chlorpromazine can also have following several:

[0139] 1. The original coating is a specific antibody, and the enzyme marker is an enzyme-labeled hapten kit

[0140] (1) The working principle of this kit is:

[0141] When pre-coating the chlorpromazine-specific antibody on the microwell strip, add the enzyme-labeled hapten solution after adding the sample solution or standard solution. The chlorpromazine or chlorpromazine standard in the sample competes with the enzyme-labeled antigen for the chlorpromazine-specific antibody coated on the microtiter plate, and the color is developed with a chromogenic solution, and the absorbance value of the sample is related to the content of chlorpromazine in the sample Become negative correlation, compare with standard curve and can draw the content of chlorpromazine in the sample. Simultaneously also can roughly judge the concentration range of chlorpromazine in the sample by co...

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Abstract

The present invention discloses a method of detecting chlorpromazine, and a special enzyme linked immunosorbent kit thereof. The enzyme linked immunosorbent kit comprises haptens and specific antibodies of the chlorpromazine; and the specific antibodies are polyclonal antibodies or monoclonal antibodies of the chlorpromazine. The kit adopts the high specificity monoclonal antibodies of the chlorpromazine, which guarantees the reliability of detection results, and the experimental results show that the kit has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like; and the main reagents of the kit all adopt the form of working liquid, the operation is convenient, and the cost is low. The operation of the method which adopts the kit to detect the chlorpromazine is simple, the operation has low requirements for the pretreatment of the sample, and the method can be used for simultaneously and rapidly detecting samples in bulk. Therefore, the method which adopts the enzyme linked immunosorbent kit for detection can perform on-site monitoring and is suitable for qualitative and quantitative screening of the samples in bulk.

Description

technical field [0001] The invention relates to a method for detecting chlorpromazine and a special ELISA kit. Background technique [0002] Chlorpromazine is a phenothiazine drug with the structural formula figure 1 As shown, it is a blocker of central dopamine polymers. This kind of medicine is added to the feed, and the livestock and poultry will sleep after eating, so as to reduce feed consumption and gain weight quickly. Human consumption of livestock and poultry products containing such drugs will also have the same effect, causing lethargy, obesity, and slow thinking. Countries such as Europe and the United States have banned or strictly banned the use of chlorpromazine. According to the No. 235 document of the Ministry of Agriculture of my country, the residue limit of chlorpromazine in food of animal origin is not allowed to be detected. Therefore, in practice, a method for detecting chlorpromazine with high accuracy and high sensitivity is needed. [0003] At p...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/577G01N33/535C12N5/12C07D279/36
Inventor 沈建忠何方洋冯才伟万宇平冯才茂汪善良刘福林赵正苗李勇
Owner BEIJING WANGER BIOTECH
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