Benzopyrene biological film deep treatment method of drinking water source
A technology of advanced treatment and benzopyrene, which is applied in the direction of sustainable biological treatment, biological water/sewage treatment, chemical instruments and methods, etc., can solve unseen problems, achieve stable performance, improve cost saving and high degradation advantages Effect
Inactive Publication Date: 2010-10-13
NANJING UNIV
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Problems solved by technology
However, there is no technical patent on the application of biofilm to advanced treatment of benzopyrene (BaP), a strong carcinogenic pollutant in drinking water sources
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Embodiment
[0046] Example: Fhhh strain biofilm reactor advanced treatment of YZNJ (Yangtze River Nanjing) drinking water source BaP.
[0047] (1) Fermentation and production of Fhhh special-effect bacterial agent: the fermentation production process parameters are shown in Table 1, and the reactor for fermentation and production of special-effect bacterial agent is B. Braun fully automatic bioreactor. Add mercaptoethanol (SH) to the Fhhh specific bacterial agent and store at 4°C.
[0048] (2) Preservation method of Fhhh special-effect bacteria agent: in the final concentration of antioxidant mercaptoethanol 0.1-0.3‰+Fe 3+ The formulation with a final concentration of 0.1-0.14% is stored at 4°C.
[0049] (3) Special-effect bacteria Fhhh biofilm reactor for advanced treatment of drinking water source BaP process implements genetically engineered bacteria biofilm reactor, biofilm reactor diameter-to-height ratio D / H≤1 / 10; carrier activated carbon volume ratio Vac=67 %; Carrier quartz sand...
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Abstract
The invention relates to a method for advanced treatment of benzopyrene of a drinking water source through a biomembrane, wherein protoplasts of eukaryotic and prokaryotic cells of three parent strains, namely white rot fungus, autochthonous bacteria YZ and Saccharomyces cerevisiae are blended, and genetic engineering bacteria are obtained through recombination and integration of genes in the same cell and construction, with a registration mark of CGMCC No.0783. The method for advanced treatment of the benzopyrene of the drinking water source by a special-effect bacterium biomembrane reactor is as follows: genetic engineering microbial inoculum is led into a column-shaped reactor which is provided with an active carbon carrier; aerated dissolved oxygen (DO) is more than 2 milligrams per liter, and domestication and membrane-forming are performed for 5 days; the biomembrane is formed on the active carbon carrier by the genetic engineering bacteria; water of the drinking water source isled into the reactor, and technological parameters of BaP are adjusted and processed, wherein the aerated dissolved oxygen is more than 2 milligrams per liter, the pH value is between 6.8 and 8.0, and the temperature is 30 +- 2 DEG C; the duty ratio is increased to 20 cubic meters per square meter per hour within 48 hours, the cell concentration reaches between 2 and 10 grams per liter, and the chemical oxygen demand (COD) removed is more than 10 percent, which shows the process of membrane-forming is successfully ended; and the stage of treatment and operation is entered, and the back washing strength on the stable operation and treatment stage is 0.2 cubic meter per square meter per second.
Description
1. Technical field [0001] The invention relates to a technical method for advanced treatment of benzopyrene (BaP) special effect bacteria Fhhh biofilm in drinking water sources. 2. Background technology [0002] There are biofilm engineering technologies for advanced treatment of drinking water at home and abroad, such as the Muirheim Water Plant in Germany; the Merisoise Water Plant in France; the Zhoujiadu Water Plant in Shanghai, China, etc. There are also strains with special effects on degrading benzopyrene [benzo(a)pyrene, BaP] in the world, such as the United States (J.Schneider, 1996) Mycobacterium sp. RJG II-135, the United States (J.A.Rentz, 2008) Sphingomonas yanoikuyae JAR02, etc. However, there is no patent for the protoplast fusion strain biofilm technology strain for advanced treatment of BaP in drinking water sources, and the advanced treatment of BaP by protoplast fusion strain is still blank. BaP is a strong carcinogenic persistent organic pollutants (pers...
Claims
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IPC IPC(8): C02F3/12
CPCY02W10/10
Inventor 程树培吴兵张宴张徐祥赵大勇崔益斌
Owner NANJING UNIV