Method for preparing anti-acetochlor soybean rhizobium inoculant

Abstract

The invention provides a method for preparing an anti-acetochlor soybean rhizobium inoculum, and belongs to a microbial inoculum preparation technology; the method uses soybean rhizobia with highly efficient nitrogen fixation activity to increase the concentration of acetochlor gradually in a sucrose yeast extract culture medium, and to culture resistant soybean rhizobia under the condition of acetochlor high-pressure selection to form a spawn of the anti-acetochlor soybean rhizobia after pure lien separation, and then selects the spawn into a sloped tube to form a primary strain, propagates the primary strain to form a secondary strain and inoculates the secondary strain to a fermentation tank to produce the microbial inoculum; the method has a simple process, low production cost, and the preparations prepared by the method has high performance and quality and can avoid damages to nitrogen fixation of soybean rhizobia by acetochlor during use and improve the yield and quality of soybean.

Description

technical field [0001] The invention belongs to a preparation technology of a soybean rhizobia agent, and mainly relates to a preparation method of an acetochlor-resistant soybean rhizobia agent. Background technique [0002] Soybean rhizobia in farmland soil infect soybean root hair epidermal cells and stimulate cell division to accelerate the formation of root nodules. Inside the nodules, rhizobia can convert nitrogen in the air into ammonia nitrogen nutrients that soybeans can use. At the same time, rhizobia use soybeans to provide nutrition for survival. Acetochlor is currently the most widely used soil treatment herbicide in soybean fields. Due to the long-term and large-scale application of acetochlor in soybean production and the unfavorable environmental conditions such as low spring temperature in northern my country's soybean production areas, it often causes damage to soybean roots and their living environment. The severe damage caused by soybean rhizobia infectin...

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems in the above-mentioned prior art, and provide a preparation method of an acetochlor-resistant soybean rhizobia agent. The acetochlor-resistant soybean rhizobia agent cultivated and produced by this method is used to inoculate soybeans to solve soybean nodules. The number of nodules and nitrogen fixation efficiency are reduced due to the influence of acetochlor, which is a problem that restricts the improvement of yield and quality in soybean production