Preparation technology of high temperature resistant feeding cellulase

A technology of cellulase and preparation process, which is applied in the direction of enzymes, enzymes, applications, etc., can solve the problems of affecting application, activity decline, enzyme protein inactivation, etc., and achieve the effect of not being resistant to high temperature

Inactive Publication Date: 2009-03-11
宝鸡市星星协力生物有限公司
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AI Technical Summary

Problems solved by technology

The optimum temperature for cellulase in feed is 40°C, and above 80°C will cause most of the enzyme protein to be inactivated, the activity will drop by 50-80%, or even have no effect
This seriously affects its application in pellet feed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] 1. High-temperature-resistant domestication of cellulase strains for production of feed:

[0015] In the first step, the cellulase solution with a concentration of 80% produced by the metabolism of the strain is placed in a water bath at 45°C, 50°C, 55°C, 60°C, 65°C, and 70°C for 5', 10', 15', use the DNS method to measure the enzyme activity respectively, and obtain the enzyme activity data of the strain after being treated at different temperatures and at different times before and after domestication, and select the strain with high temperature resistance for a relatively long time as the initial strain;

[0016] In the second step, the ultraviolet irradiation method is used for mutagenesis, and the bacterial strains are exposed to ultraviolet lamps for 30', 60', and 90' respectively, and the bacterial strains with different irradiation times are taken for multiplication culture, dilution and separation, and a single colony is obtained, and then Select the colonies, ...

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PUM

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Abstract

The invention relates to a process for preparing cellulase used for heat resisting feeds. The process comprises the following steps: screening out heat resisting strains for preservation by performing heat resisting domestication first on the strains for the cellulase used for feed production, and performing transfer of culture on the preserved strains; and then fermenting the screened heat resisting strains to prepare into the mixed enzyme liquid, and concentrating the mixed enzyme liquid to certain concentration to perform microcapsule coating. The process can improve the tolerance temperature of the cellulase used for the feeds from 65 DEG C keeping for 10 minutes to 85 DEG C keeping for 10 minutes, and basically solve the difficult problem that the cellulase used for the feeds does not resist high temperature, thereby laying the technical foundation for the wide application.

Description

technical field [0001] The invention relates to a preparation process of high temperature resistant feed cellulase Background technique [0002] Feed cellulase, as a kind of feed enzyme preparation, can greatly increase the utilization rate of non-starch polysaccharides in feed, save feed, promote animal growth and improve its production performance. Its application effect has been widely used by feed enterprises and farms. confirmed. However, some inherent defects of enzyme preparations have affected its promotion to a wider range of fields, and cellulase for feed is no exception. Among many influencing factors (such as: strain source, fermentation temperature, humidity, pH, time, processing technology, storage conditions, etc.), the feed processing technology has a great influence on the effect of feed cellulase, especially some feed (eg: pellet feed) requires a "high temperature pelleting" process (generally 80-90°C, 10 minutes). The optimal temperature for cellulase i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42A23K1/165A23K20/189
Inventor 王刚王平王焕高刘丽
Owner 宝鸡市星星协力生物有限公司
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