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Preparation method of buckwheat protein biological activity peptides

A bioactive peptide, buckwheat protein technology, applied in the direction of fermentation, can solve the problems of affecting digestion and absorption, low sensitivity of protease, late research on active peptides, etc., and achieve the effect of simple preparation method

Inactive Publication Date: 2009-03-11
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Buckwheat is rich in protein and has an appropriate proportion of essential amino acids, but its digestion and absorption utilization rate is low, which is related to the presence of antinutritional factors (such as trypsin inhibitors, tannins, etc.) in buckwheat seeds and the low sensitivity of buckwheat protein to proteases. related
In addition, dietary fiber, polysaccharides, phytic acid, and high-concentration rutin also have inhibitory effects on trypsin activity, thereby affecting digestion and absorption
However, the research on the hydrolysis of buckwheat protein and its hydrolyzate active peptides is relatively late, and there is no comprehensive and in-depth discussion.

Method used

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  • Preparation method of buckwheat protein biological activity peptides

Examples

Experimental program
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Effect test

Embodiment 1

[0018] 1) Dissolve buckwheat protein in NaOH solution of pH 6.0 to make the protein concentration reach 15g / L;

[0019] 2) At 40°C, add 4000U / g alkaline protease, hydrolyze for 2h, and the degree of hydrolysis is 40%;

[0020] 3) After the hydrolyzed protein peptide solution was incubated in a 95° C. water bath for 10 min to inactivate the enzyme, centrifuged at 10,000 rpm for 40 min, and the clear liquid was vacuum freeze-dried to obtain dry powder of buckwheat protein bioactive peptide.

Embodiment 2

[0022] 1) Dissolve the buckwheat protein in the NaOH solution of pH 8.0 to make the protein concentration reach 18g / L;

[0023] 2) At 30°C, add 5000U / g alkaline protease, hydrolyze for 1.5h, and the degree of hydrolysis is 30%;

[0024] 3) After the hydrolyzed protein peptide solution was incubated in a 97° C. water bath for 10 min to inactivate the enzyme, centrifuged at 10,000 rpm for 40 min, and the clear liquid was vacuum freeze-dried to obtain dry powder of buckwheat protein bioactive peptide.

Embodiment 3

[0026] 1) Dissolve buckwheat protein in NaOH solution of pH 12.0 to make the protein concentration reach 21g / L;

[0027] 2) At 50°C, add 6500U / g alkaline protease, hydrolyze for 3h, and the degree of hydrolysis is 55%;

[0028] 3) After the hydrolyzed protein peptide solution was incubated in a 96° C. water bath for 10 min to inactivate the enzyme, centrifuged at 10 000 rpm for 40 min, and the clear liquid was vacuum freeze-dried to obtain dry powder of buckwheat protein bioactive peptide.

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Abstract

The invention discloses a method for preparing buckwheat protein bioactive peptide. The method comprises the following steps: buckwheat proteins are dissolved into a NaOH solution with a pH value between 6.0 and 12.0, so as to make the concentration of the proteins reach between 15 and 21 grams per liter; 4,000 to 65,000 units per gram of alkaline protease is added at a temperature of between 30 and 50 DEG C for hydrolysis for 1 to 3 hours, and the hydrolysis degree is between 30 and 55 percent; a protein peptide solution after hydrolysis undergoes heat preservation for 10 minutes in a water bath at a temperature of between 95 and 100 DEG C; after passivation of an enzyme, the protein peptide solution is centrifuged for 40 minutes at the rotating speed of 10,000 revolutions per minute; and after vacuum freeze drying of a clear solution, drymeal of the buckwheat protein bioactive peptide is obtained. The preparation method is simple and is suitable for industrial mass production.

Description

technical field [0001] The present invention relates to a preparation method of buckwheat protein biologically active peptide. Background technique [0002] The polypeptides obtained by enzymatic hydrolysis of proteins are more easily absorbed by the human body than the original proteins and amino acids. These polypeptides have various biological activities, such as promoting immunity, hormone regulation, antibacterial, antioxidant, antiviral, lowering blood pressure, lowering blood lipids and other functions. Using various proteins as raw materials and selecting enzymes suitable for their hydrolysis to obtain polypeptides with various nutritional and functional properties has become one of the important directions of protein development and utilization. [0003] Buckwheat is rich in protein and has a suitable ratio of essential amino acids, but its digestion and absorption utilization rate is low, which is related to the existence of anti-nutritional factors (such as trypsi...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 高梅张国权罗勤贵丰凡
Owner NORTHWEST A & F UNIV