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Plant salt tolerant protein and encoding gene and application thereof

A technology that encodes genes and plants, applied in the direction of plant gene improvement, application, plant peptides, etc., can solve problems such as limited economic value, and achieve the effect of improving salt tolerance and high application value

Active Publication Date: 2009-03-18
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most crops are very sensitive to salt. Although halophytes can survive under high-salt conditions, their economic value is often very limited. Improving the salt-resistant ability of crops has become an important content in agricultural production and scientific research in my country

Method used

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  • Plant salt tolerant protein and encoding gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the acquisition of plant salt tolerance protein and its coding gene

[0030] The ESTs obtained from the cDNA library constructed from the leaves of soybean variety Suinong 14 were analyzed, and an EST with Na + / H + Antiporter genes have high homology. The full-length cDNA sequence of the gene was obtained from Wenfeng No. 7 (unified number: ZDD02611; China National Germplasm Bank) treated with salt for 6 h by RT-PCR and 3' RACE. The specific method is as follows:

[0031] Soak the roots of soybean variety Wenfeng 7 seedlings grown for 2 weeks after germination with 200mM NaCl solution for 6 hours, harvest the leaves to extract RNA and reverse transcribe it into the first strand of cDNA, the first strand of cDNA is synthesized according to the ReverseTranscription System Kit (Promega) According to the instructions of the kit, the cDNA was used as a template to carry out PCR amplification with primers N21F (5'-CAGTTGTGTTGAGGGACACA-3') and N21R (5'-GCACTCG...

Embodiment 2

[0041] Example 2, Functional Verification of Plant Salt Tolerance Protein and Its Encoding Gene

[0042] 1. Construction of overexpression vector in Arabidopsis

[0043] The ORF of GmNHX2 was cloned into the pBI121 vector and placed under the control of the 35S promoter. The specific method is:

[0044] Primers were designed according to the above GmNHX2 cDNA sequence, the sequence is as follows:

[0045] BamN2: 5'-GCC GGATCC ATGGCCTCGGAATTAGAAATTTC-3' (underlined is the BamH I enzyme recognition site);

[0046] SacN2: 5′-GCG GAGCTC TCATGATGAATAGTGGTTCTGGC-3' (the Sac I enzyme recognition site is underlined).

[0047] The roots of soybean variety Wenfeng 7 seedlings grown for 2 weeks after germination were soaked in 200mM NaCl solution for 6 hours. RNA was extracted from the leaves and cDNA was obtained by reverse transcription. As a template, PCR amplification was performed using BamN2 and SacN2 ​​as primers.

[0048] A fragment of 1605bp was amplified by PCR, and seq...

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Abstract

The invention discloses a vegetable salt resistant protein and a coding gene and application thereof. The vegetable salt resistant protein is a protein having one of the following amino acid residue sequences: 1) the SEQ ID No. 2 amino acid residue sequence in a sequence table; and 2) a protein which has vegetable salt resistance and is obtained through replacing and / or deleting and / or adding one or a plurality of amino acid residues in and / or from and / or into the SEQ ID No. 2 amino acid residue sequence in the sequence table. Experiments show that the protein has high salt resistance, and the vegetable salt resistance can be improved through shifting coding genes of the protein into vegetables.

Description

technical field [0001] The invention relates to a plant salt-tolerant protein and its coding gene and application. Background technique [0002] The threat of soil salinization to agriculture is a global problem. According to statistics, there are about 1 billion hectares of saline-alkali soil in the world, accounting for nearly 7.6% of the world's land area. my country is a developing agricultural country, and there are more than 20 million hectares of saline-alkali soil in the country, accounting for 1 / 3 of the country's cultivated land area. Salt stress can significantly inhibit the growth of plants, and even lead to the death of plants. In the long-term evolutionary process, different plants have formed different mechanisms to adapt to salt stress, and their salt resistance capabilities are also different. Most crops are very sensitive to salt. Although halophytes can survive under high-salt conditions, their economic value is often very limited. Improving the salt-res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H1/00A01H5/00
Inventor 周国安邱丽娟李英慧关荣霞刘章雄金龙国张丽娟王克晶李向华常汝镇
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI