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Plant salt stress tolerant protein and encoding gene and application thereof

A plant stress tolerance and stress tolerance technology, applied in the direction of plant genetic improvement, application, plant peptides, etc., can solve problems such as limited economic value, and achieve the effect of improving salt tolerance and drought resistance, and high application value.

Active Publication Date: 2012-08-29
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most crops are very sensitive to salt. Although halophytes can survive under high-salt conditions, their economic value is often very limited. Improving the salt-resistant ability of crops has become an important content in agricultural production and scientific research in my country

Method used

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  • Plant salt stress tolerant protein and encoding gene and application thereof
  • Plant salt stress tolerant protein and encoding gene and application thereof
  • Plant salt stress tolerant protein and encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the acquisition of plant stress tolerance protein and its coding gene

[0032] The ESTs obtained from the cDNA library constructed from the leaves of soybean variety Suinong 14 were analyzed, and an osmotic stress-related EST was found. The tblast program was used to search the GenBank database, and a pair of primers were designed. A cDNA of a plant stress tolerance protein was cloned from leaves of Feng 7 (unified number: ZDD02611; provided by China National Germplasm Bank). The specific method is:

[0033] Soak the roots of soybean variety Wenfeng 7 seedlings grown for 2 weeks after germination with 200mM NaCl solution for 6 hours, harvest the leaves, extract RNA and reverse transcribe it into the first strand of cDNA. Using the cDNA as a template, primer PK1F(5' -TGCTACGCACACACAAAACACA-3′) and PK1R

[0034] (5'-TATGTCCCATGCGGAAAACTT-3') for PCR amplification.

[0035] The PCR reaction system is: 10×Buffer2.5μl, dNTP2.5mM, PK1F5μM, PK1R5μM, cDNA20ng, ...

Embodiment 2

[0038] Example 2, Functional Verification of Plant Stress Tolerance Protein and Its Encoding Gene

[0039] 1. Construction of overexpression vector in Arabidopsis

[0040] Clone the GmPK ORF into the pBI121 vector and place it under the control of the 35S promoter. The specific method is:

[0041] Primers were designed according to the above GmNHX2 cDNA sequence, the sequence is as follows: F1: 5'-CGC GTC GAC ATGGAGAAATACGAGCTCGT-3' (underlined is the Sal I enzyme recognition site); R1: 5'-CTG CTCGA GTTAACTGACATGAAATTCCC-3' (Xho I enzyme recognition site is underlined).

[0042]Soak the roots of soybean variety Wenfeng 7 seedlings grown for 2 weeks after germination in 200mM NaCl solution for 6 hours, extract RNA from the leaves and reverse transcribe to obtain the first-strand cDNA, using the cDNA as a template and using F1 and R1 as primers Perform PCR amplification.

[0043] A fragment of 1077bp was amplified by PCR, and sequencing showed that the fragment had the nuc...

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Abstract

The invention discloses a vegetable adversity resistant protein and a coding gene and application thereof. The vegetable adversity resistant protein is a protein having one of the following amino acid residue sequences: 1) the SEQ ID No. 2 amino acid residue sequence in a sequence table; and 2) a protein which has vegetable salt resistance and is obtained through replacing and / or deleting and / or adding one or a plurality of amino acid residues in and / or from and / or into the SEQ ID No. 2 amino acid residue sequence in the sequence table. Experiments show that the protein has high resistance tosalt and drought and the vegetable resistance to salt and drought can be improved through shifting coding genes of the protein into vegetables.

Description

technical field [0001] The invention relates to a plant stress tolerance protein and its encoding gene and application. Background technique [0002] The threat of soil salinization to agriculture is a global problem. According to statistics, there are about 1 billion hectares of saline-alkali soil in the world, accounting for nearly 7.6% of the world's land area. my country is a developing agricultural country, and there are more than 20 million hectares of saline-alkali soil in the country, accounting for 1 / 3 of the country's cultivated land area. Salt stress can significantly inhibit the growth of plants, and even lead to the death of plants. In the long-term evolutionary process, different plants have formed different mechanisms to adapt to salt stress, and their salt resistance capabilities are also different. Most crops are very sensitive to salt. Although halophytes can survive under high-salt conditions, their economic value is often very limited. Improving the sal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H1/00A01H5/00
Inventor 周国安邱丽娟李英慧关荣霞刘章雄金龙国张丽娟王克晶李向华常汝镇
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI