Prevention/treatment of ichthyosis vulgaris, atopy and other disorders

A technology of diseases and reagents, applied in gene therapy, microbiological determination/examination, skin diseases, etc., can solve problems such as unidentified genome mutations

Inactive Publication Date: 2009-04-01
UNIV COURT OF THE UNIV OF DUNDEE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although biochemical analysis revealed defective profilaggrin operation in ft / ft homozygotes, no genomic mutations in the FLG gene have been identified to date

Method used

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  • Prevention/treatment of ichthyosis vulgaris, atopy and other disorders
  • Prevention/treatment of ichthyosis vulgaris, atopy and other disorders
  • Prevention/treatment of ichthyosis vulgaris, atopy and other disorders

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: Co-reporter gene constructs for testing read-through reagents

[0044] The oligonucleotide cassette corresponding to base numbers 1480-1523 of the human filaggrin gene, (FLG; coding sequence numbered starting from the ATG start codon; Genbank accession number NM_002016.1), was cloned into the unique Nco I restriction This site spans the ATG codon of the firefly luciferase gene (f-Luc) in the plasmid vector pSP-luc+NF (Promega). figure 2 The oligonucleotide cassette shown in has an added overhang corresponding to the cohesive end of Nco I. These boxes correspond to the region of the human FLG gene containing codon 501, the common R501X mutation site. Wild type and R501X mutant forms were formed. Clones with inserts in the correct orientation were identified and verified by DNA sequencing. After insertion of the FLG cassette, the ATG codon, the original start codon of the f-Luc gene, was mutated to an AGG arginine codon by site-directed mutagenesis using the...

Embodiment 2

[0047] Example 2: Generation of Human Suppressor tRNA Genes for TGA Nonsense Mutations

[0048] The human genome includes a large number of CGA-arginine t-RNA genes. These are very compact genes and contain an intragenic promoter for RNA polymerase III (Lewin B., In: Genes VII, Oxford University Press, 2000, pp. 624-626). Two of these genes were identified in the current collection of the human genome (HG17; http: / / genome.ucsc.edu), named TR3 and TR4B for convenience, and amplified from normal human control DNA . TR3 is located on chromosome 6p22.1 and TR4B is located on chromosome 15q26.1. Specifically, the TR3 gene was amplified as a 668 bp fragment, which corresponds to the human genome HG17 collection, using primers TR3.F 5′ CGA TGC AGA CAATATGCA GA 3′ and TR3.R 5′ CTAAGC CTA CAA AAC CGA AA 3′ The base number chr6:26,407,555-26,408,284. The TR4B gene was amplified as a 465 bp fragment using primers TR4B.F 5'GAC TTC TGG GTG GGCTCT CC 3' and TR4B.R 5'CCC TCC CTC TCC ACT ...

Embodiment 3

[0049] Example 3: Dual Luciferase Assay for FLG Readthrough

[0050] The co-reporter constructs pRLF-FLG-WT and pRLF-FLG-501X were transiently transfected into 293 cells (transformed human kidney epithelial cell line) in 96-well plates by utilizing the Fugene-6 system (Roche) , and test them. Forty-eight hours after transfection, the cells were subjected to the Dual Luciferase Assay System (Promega), which detects specific luciferase signals corresponding to Renilla and Firefly luciferase reporters. For the wild-type co-reporter gene, pRLF-FLG-WT, a strong signal for Renilla and firefly luciferase was obtained, as shown in Figure 6 as shown in a. In contrast, only the Renilla signal was obtained for the pRLF-FLG-501X construct, showing that the R501X premature stop codon mutation present within the FLG box in this construct resulted in complete loss of firefly luciferase expression ( Figure 6 a).

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Abstract

The present invention relates to the prevention / treatment of ichthyosis vulgaris (IV), atopy and potentially other disorders associated with loss-of-function mutations in the filaggrin gene sequence. The prevention / therapy is based on the use of agents which enable the host's translational machinery to read through a nonsense mutation found in a mutant allele of the filaggrin gene.

Description

field of invention [0001] The present invention relates to the prevention / treatment of ichthyosis vulgaris (IV), atopy and potentially other conditions associated with loss-of-function mutations in the filaggrin gene sequence. This prophylaxis / treatment is based on the use of agents that enable the host's translation system to read through the nonsense mutation present in the mutant allele of the filaggrin gene. Background of the invention [0002] Ichthyosis vulgaris (IV; OMIM# 146700) is the most prevalent genetic disorder of keratosis and one of the most common monogenic disorders in humans. The most widely cited incidence figure is 1 in 250, based on a survey of 6051 healthy British schoolchildren 1 . The relationship between IV and atopy is well established; 37-50% of people with IV have atopy, specifically atopic dermatitis (eczema) 1，15 , and conversely, approximately 8% of patients with atopic dermatitis have typical IV features 1，16 . [0003] Phenotypic featur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47A61K31/7036A61K48/00C12Q1/68
CPCA61K31/7036C07K14/4713Y10T436/143333A61P11/06A61P17/00A61P17/04A61P17/06A61P17/16A61P37/08
Inventor 威廉姆·亨利·欧文·麦克莱恩弗朗西斯·简·多罗西·史密斯
Owner UNIV COURT OF THE UNIV OF DUNDEE
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