Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Reagent for detection of pathogenic hydrophila gingivalis and detection method thereof

A technology for detection of Aeromonas hydrophila and a detection method, which is applied in the field of reagents for detection of pathogenic Aeromonas hydrophila, can solve problems such as time-consuming, cumbersome operation, and difficulty in grasping, and achieve simple operation, strong specificity, easy to get effect

Inactive Publication Date: 2009-04-29
INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
View PDF0 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is relatively cumbersome to operate, and the data processing of the experimental results requires artificial comparison and judgment of the colony morphology and biochemical characteristics. The accuracy often varies from person to person, difficult to grasp, and time-consuming

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reagent for detection of pathogenic hydrophila gingivalis and detection method thereof
  • Reagent for detection of pathogenic hydrophila gingivalis and detection method thereof
  • Reagent for detection of pathogenic hydrophila gingivalis and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] Randomly take 6 water samples from the water body of a breeding pond of a certain eel factory in Fujian Province, separate the pathogenic bacteria, take 30ml water samples each, centrifuge, 1000rpm (1000 revolutions per minute) for 5mins, take the supernatant, 3900rpm Centrifuge for 30mins, discard the supernatant, suspend the precipitate with 2ml TE buffer, take a small amount of the suspension in a test tube containing 5ml sterilized alkaline peptone water, incubate at 30°C for 15 hours, then streak and inoculate on an ordinary agar plate, incubate at 30°C for 15 hours Hour.

[0022] Then carry out strain culture: the standard strain of Aeromonas hydrophila was cultured in common broth based on 30° C., 200 rpm shaking culture for 15 hours.

[0023] Preparation of template DNA: Take 1.5ml of the above-mentioned bacterial solution, separate it with a centrifugal force of 15000g for 10 minutes, and collect the bacterial precipitate. Suspend the bacterial pellet with 150...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a reagent for detecting pathogenic aeromonas hydrophila, which comprises the following solutions: 1 milliliter of 10xPCR buffer solution, 60 microliters of Pf solution, 60 microliters of Pr solution, 1 milliliter of 2xPCR water and 30 microliters of Taq enzyme. The invention also relates to a detection method which applies the reagent for detecting the pathogenic aeromonas hydrophila. The reagent has the characteristics of high sensitivity, strong specificity and easily obtained template materials, and the detection method has the characteristics of simple and convenient operation and quickness and time conservation.

Description

technical field [0001] The invention relates to a reagent for detecting pathogenic Aeromonas hydrophila and a detection method thereof. Background technique [0002] Aeromonas hydrophila (A.hydrophila) widely exists in fresh water and water containing organic matter, silt and fresh water fish body surface. Aeromonas hydrophila can be divided into pathogenic strains and non-pathogenic strains. Pathogenic Aeromonas hydrophila can produce highly toxic exotoxins, such as: hemolysin (hemolysin), tissue toxin, necrotic toxin, enterotoxin and protease. Pathogenic strains can infect animals such as fish, amphibians, reptiles, birds and mammals. It can cause a variety of fish infectious diseases, especially red fin disease, fulminant hemorrhagic disease, and sepsis, which cause a large number of eels, and the mortality rate is very high. At the same time, Aeromonas hydrophila can also cause human diseases, such as acute gastroenteritis in people of different ages. The current met...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12Q1/04
Inventor 李寿崧林俊生江树勋饶静静江云陈文炳邵碧英黄晓蓉
Owner INSPECTION & QUARANTINE TECH CENT OF FUJIAN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com