Ginkgo biloba leaf extract having novel composition useful for prevention or treatment of alzheimer's disease and parkinson's disease, and extracting and purifying method thereof

A technology for Alzheimer's disease and Parkinson's disease, applied in drug combinations, medical raw materials derived from ginkgo biloba, nervous system diseases, etc.

Active Publication Date: 2009-05-13
株式会社柳柳制药
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, Ginkgo biloba extract with activity superior to standardized Ginkgo biloba extract (DAB) has not been developed until now, and how to directly extract and purify the above extract from Ginkgo biloba has not been reported yet

Method used

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  • Ginkgo biloba leaf extract having novel composition useful for prevention or treatment of alzheimer's disease and parkinson's disease, and extracting and purifying method thereof
  • Ginkgo biloba leaf extract having novel composition useful for prevention or treatment of alzheimer's disease and parkinson's disease, and extracting and purifying method thereof
  • Ginkgo biloba leaf extract having novel composition useful for prevention or treatment of alzheimer's disease and parkinson's disease, and extracting and purifying method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The dry powder (10kg) of Ginkgo biloba is placed in a container, and 100L of acetone aqueous solution (60% by volume) is added for extraction. After 12 hours, pressure filtration was carried out, and the filtrate was concentrated to 10 L under reduced pressure. The concentrate was diluted to 20 L with water and vigorously agitated. Thereafter, the diluted solution was left at 15° C. for 12 hours, and the supernatant was separated from the precipitate, followed by filtration. The obtained filtrate was concentrated under reduced pressure and dried, whereby 252 g of ginkgo leaf extract were prepared.

[0045] Example 1

[0046] The dry powder (5kg) of ginkgo biloba is placed in a container, and 50L of ethanol aqueous solution (50% by volume) is added for extraction. After 12 hours, pressure filtration was carried out, and the filtrate was concentrated to 5 L under reduced pressure. The concentrate was diluted to 10 L with water and stirred vigorously. Thereafter, the ...

Embodiment 2

[0048] Ginkgo biloba extract 100g prepared in comparative example 1 was dissolved in 1L ethanol aqueous solution (50v / v%). The solution was treated three times with 500 ml of hexane, and the hexane-soluble fraction was removed. The remaining aqueous layer was treated three times with 1 L of a mixed solvent of butanol and toluene (1:1, v / v). The mixed solvent layer was left to stand at 15° C. for 12 hours and then filtered using filter paper. The remaining solution was concentrated under reduced pressure and dried, whereby 54.7 g of a purified ginkgo leaf extract were obtained.

Embodiment 3

[0050] The dry powder (5kg) of ginkgo biloba is placed in a container, and 50L of ethanol aqueous solution (50% by volume) is added for extraction. After 12 hours, it was filtered under pressure, and the filtrate was concentrated to 5 L under reduced pressure. The concentrate was diluted to 10 L with water and stirred vigorously. Thereafter, the resulting diluted solution was left at 15° C. for 12 hours, and the supernatant was separated from the precipitate, followed by filtration. The aqueous layer thus obtained was treated 3 times with 5 L of hexane, and the hexane-soluble portion was removed. The remaining aqueous layer was treated three times with 10 L of butanol and toluene (1:1, v / v) mixed solvent. The mixed solvent layer was left to stand at 15° C. for 12 hours and then filtered using filter paper. The remaining solution was concentrated under reduced pressure and dried, whereby 48.7 g of a purified ginkgo leaf extract were obtained.

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PUM

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Abstract

The present invention relates to a ginkgo biloba leave extract comprising 10-14 wt% (preferably 11-13 wt% and most preferably 12 wt%) of a terpene trilactone, 20-30 wt% of a ginkgo flavone glycoside and less than 5 ppm of a ginkgolic acid relative to total weight of the extract, which is superior to the conventional ginkgo biloba leave extract comprising the standardized ginkgo biloba extract (German pharmacopoeia 2000) in inhibiting the generation of NO (nitric oxide) and treating Alzheimer's disease and Parkinson's disease, and the present invention also relates to the extraction and purification method thereof and a pharmaceutical composition comprising the same as an active ingredient.

Description

technical field [0001] The present invention relates to a Ginkgo biloba extract comprising 10-14% by weight (preferably 11-13% by weight and most preferably 12% by weight) of terpene lactones, 20-30% by weight of Ginkgo flavonoid glycosides and less than 5ppm ginkgolic acid, which is superior to conventional formulations containing standard Ginkgo biloba extract (German Pharmacopoeia 2000) in inhibiting NO (nitric oxide) production and treating Alzheimer's and Parkinson's diseases Ginkgo biloba extract, and the present invention also relates to its extraction and purification methods and pharmaceutical compositions containing it as an active ingredient. Background technique [0002] Ginkgo biloba extract is effectively used in the treatment of peripheral arterial occlusive disease, vertigo, vascular tinnitus and degenerative tinnitus, organic encephalopathy syndrome, and has been used as a drug treatment in Germany, France and the current world. Ginkgo biloba extract contai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P25/28
CPCA61K36/16A61P25/28
Inventor 金滢春金显杓李京姬李龙五金裕鲜金暎河洪准基
Owner 株式会社柳柳制药
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